Term
|
Definition
| Gram-reaction of Gram+ bacteria |
|
|
Term
|
Definition
| Gram-reaction of Gram- Bacteria |
|
|
Term
|
Definition
| Sensor protein in the inner membrane, part of two-component regulatory system |
|
|
Term
|
Definition
| Functions as a DNA binding protein, transcriptional regulator |
|
|
Term
| Transcriptional regulator |
|
Definition
| Binds to promoter, starts transcription |
|
|
Term
|
Definition
| Product of phosphorylation of ATP in two component regulatory system, can now bind to promoter region |
|
|
Term
| 5' UTR (Untranslated Region) |
|
Definition
| Stops transcription when Mg+ levels are high by changing confirmation. |
|
|
Term
| Flux of genetic information |
|
Definition
| Replication->Transcription->Translation |
|
|
Term
| First step of DNA replication |
|
Definition
| Double-stranded DNA unwinds |
|
|
Term
|
Definition
| Synthesis of DNA single strand by DNA pol |
|
|
Term
|
Definition
| Helicase unwinds parental double helix |
|
|
Term
|
Definition
| Binding proteins stabilize separate strands |
|
|
Term
|
Definition
| Primase adds short primer to template strand |
|
|
Term
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
| RNA Pol binding, transcription of gene |
|
|
Term
| Transcription initiation site is located |
|
Definition
| +1 downstream from promoter |
|
|
Term
|
Definition
| Provides a binding site for transcriptional regulator and repressor |
|
|
Term
|
Definition
| Asymetric, only one strand is copied |
|
|
Term
| Sequence that matches 5'-3' mRNA sequence |
|
Definition
|
|
Term
| E.coli RNA pol consists of |
|
Definition
|
|
Term
| Core enzyme + Sigma Factor = |
|
Definition
|
|
Term
| Holoenzyme is required for correct initiation of transcription by |
|
Definition
|
|
Term
| 6S RNA mimicking the open complex results in |
|
Definition
| Trapping RNA pol, stopping transcription. |
|
|
Term
| Rho-dependent termination |
|
Definition
|
|
Term
| Rho-Independent Termination |
|
Definition
|
|
Term
|
Definition
| Collection of genes or operons under regulation by the same regulatory protein |
|
|
Term
|
Definition
| Collection of genes under regulation by the same stimulus |
|
|
Term
|
Definition
| Technique to measure level of induced mRNA |
|
|
Term
|
Definition
| Technique to measure level of induced mRNA |
|
|
Term
|
Definition
| Method to measure level of induced protein |
|
|
Term
| Hybridization - Synthesis of cDNA using RNA as a template |
|
Definition
|
|
Term
|
Definition
| An array of known DNA fragments fixed to the surface of a supporting chip |
|
|
Term
| How does a DNA microarray work? |
|
Definition
| It measures expression levels of large numbers of genes simultaneously. |
|
|
Term
|
Definition
| Used to label cDNA in a microarray, acts as an electrophoresis dye |
|
|
Term
|
Definition
| 2'3'-Dideoxyadenosine triphosphate, They act as chain terminators so when added by the polymerase, elongation is halted. |
|
|
Term
|
Definition
| Breaks down thymine dimers |
|
|
Term
|
Definition
| In order to inactivate LexA repressor |
|
|
Term
|
Definition
|
|
Term
|
Definition
| DNA damage, is created to generate gene expression |
|
|
Term
|
Definition
| Post-replication DNA repair system, relies on a repressor to be removed to initiate. |
|
|
Term
| Lytic or Virulent (Bacteriophage) |
|
Definition
| Phage that multiplies within the host cell, lyse the cell and release progeny phage. |
|
|
Term
| Lysogenic or Temperate Phage |
|
Definition
| Phage that can either multiply via the lytic cycle or enter a quiescent state in the bacterial cell. |
|
|
Term
|
Definition
| Expression of most phage genes repressed |
|
|
Term
|
Definition
| Required for the binding for the sugar maltose, is a surface protein |
|
|
