Term
| Mannitol Salt Agar (MSA) is SELECTIVE for __________, because of _________. |
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Definition
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Term
| Mannitol Salt Agar (MSA) is DIFFERENTIAL because of _________. |
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Definition
| the carbohydrate mannitol and the pH indicator phenol red |
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Term
| In Mannitol Salt Agar (MSA), a yellow zone around a bacterial colony indicates what? |
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Definition
| Mannitol fermentor. The fermentation creates an acidic byproduct, which appears as yellow. |
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Term
| In Mannitol Salt Agar (MSA), a yellow zone around a bacterial colony indicates what? |
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Definition
| Mannitol fermentor. The fermentation creates an acidic byproduct, which appears as yellow. |
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Term
| What color would the agar under a NON mannitol fermenting bacteria appear on Mannitol Salt Agar (MSA)? |
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Definition
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Term
| What bacteria is coagulase important for? |
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Definition
| Pathogenic staphylococci. |
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Term
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Definition
| Binds plasma fibrogen to clot plasma. |
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Term
| What does the esculin test determine? |
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Definition
| The ability to hydrolyze esculin to glucose and esculetin. |
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Term
| How would a A/A KIA test appear, and what does that mean? |
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Definition
| Pure yellow tube. This is lactose fermenting. |
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Term
| How would a K/A KIA test appear, and what does it mean? |
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Definition
| Yellow deep, pink slant. Non-lactose fermenting. |
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Term
| MacConkey's Agar (MAC) is SELECTIVE because of _______________ which inhibits most _________. |
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Definition
| crystal violet and bile salts, gram positive bacteria. |
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Term
| MAC agar is differential because of __________, which determines the ability to _________. |
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Definition
| the carbohydrate lactose and the pH indicator neutral red, ferment lactose. |
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Term
| On MAC agar, what would a pink zone indicate? |
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Definition
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Term
| True or false? All Enterobacteriaceae ferment lactose. |
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Definition
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Term
| True or false? Not all Enterobacteriaceae can ferment lactose. |
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Definition
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Term
| What three bacilli ferment lactose, and what are they called as a group? |
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Definition
| Coliform bacilli. Klebsiella spp., E. coli, and Enterobacter spp. |
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Term
| In Brilliant Green agar, what does a yellow/green zone indicate? |
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Definition
| Sugars are fermented, producing an acidic byproduct. |
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Term
| What sugars does Brilliant Green agar contain? |
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Definition
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Term
| Salmonella appears _______ on Brilliant Green agar becacuse ________. |
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Definition
| pink, it does not have the ability to ferment sugars. |
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Term
| In a LIA test, what does a K/K reaction look like? |
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Definition
| The tube is totally purple. |
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Term
| In a LIA test, what does a completely purple tube indicate? |
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Definition
| A K/K reaction. Glucose is fermented, then lysine decarboxylase is produced which convers lysine to cadavarine, thus turning the agar purple. POSITIVE REACTION |
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Term
| In a LIA test, what does a K/A tube look like? |
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Definition
| Purple slant, yellow deep. |
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Term
| In a LIA test, what does a purple slant and a yellow deep indicate? |
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Definition
| K/A reaction. Negative for lysine decarboxylase. |
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Term
| What would most salmonella look like in a LIA test? |
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Definition
| K/K, pure purple tube. Lysine decarboxylase POSITIVE |
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Term
| Describe E. coli on a plate of agar. |
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Definition
| Medium/large, white, shiny |
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Term
| E. coli is oxidase ___, and can/cannot ferment lactose. |
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Definition
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Term
| Neisseria is a gram __ __. |
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Definition
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Term
| Salmonella is a gram __ __. |
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Definition
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Term
| Pseudomonas is a gram __ __. |
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Definition
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Term
| Corynedipheria is gram __ and looks like _____ under a microscope. |
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Definition
| positive, chinese letters |
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Term
| Bacillus cereus are long/short gram ___ ___. |
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Definition
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Term
| Listeria are long/short gram __ __. |
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Definition
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Term
| Streptococcus pneumonia are gram __ __ and are often found in (chains, pairs, alone, etc)___. |
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Definition
| gram positive cocci, pairs |
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Term
| Staphylococcus aureus are gram __ __. |
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Definition
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