Term
|
Definition
| Non-fastidious organisms. Examples of fastidious organisms (i.e. ones which won't grow on TSA) are Neisseria and Hemophilus. |
|
|
Term
|
Definition
| Enriched, allows growth of fastidious organisms like Hemophilus and Neisseria. (Also non-fastidious bugs will grow too!) |
|
|
Term
|
Definition
| Selective for Neisseria, good for using on cultures that contain a lot of microflora like vaginal or throat swabs. It's Chocolate Agar with Colistin (kills most Gram neg)Vancomycin(kills Gram +), and Nystatin (kills fungi). |
|
|
Term
|
Definition
| Selects for non-fastidious gram negatives and is differential for lactose fermentation. |
|
|
Term
| What do red and white colonies mean, respectively, on MacConkey agar? |
|
Definition
| Red: Lactose fermenter White: Non-lactose fermenter |
|
|
Term
| What color would E.coli be on MacConkey agar? |
|
Definition
|
|
Term
| What color would Salmonella enteridis be on MacConkey agar? |
|
Definition
|
|
Term
| Eosin-methylene blue agar |
|
Definition
| Differentiates lactose fermenters from non-lactose fermenters. |
|
|
Term
| What are blue/black colonies on Eosin-methylene blue agar |
|
Definition
| Lactose fermenters (e.g. E.coli, Klebsiella) |
|
|
Term
| What are colorless or light purple colonies on Eosin-methylene blue agar? |
|
Definition
| Non-lactose fermenters (e.g. salmonella, shigella) |
|
|
Term
|
Definition
| Detects lactose fermentation, H2S production, inhibits non-enterics |
|
|
Term
| What are Yellow or salmon colonies on Hektoen Agar? |
|
Definition
| lactose fermenters (i.e. E.coli) |
|
|
Term
| What are colorless colonies on Hektoen agar? |
|
Definition
| Non-lactose fermenters (e.g. Salmonella, Shigella) |
|
|
Term
| What does a black precipitate indicate on Hektoen agar? |
|
Definition
| Hydrogen Sulfide (H2S) production. (e.g. Salmonella) |
|
|
Term
|
Definition
| Selects for Staphylococci and differentiates Staphylococcus Aureus from other species. |
|
|
Term
| What is a yellow colony on Mannitol Salt Agar? |
|
Definition
| Staphylococcus Aureus (ferments mannitol) |
|
|
Term
| What is a white colony on Mannitol Salt Agar? |
|
Definition
| Staphylococcus sp. that is not Staph Aureus. |
|
|
Term
|
Definition
| Detects glucose, lactose, sucrose fermentation; gas and H2S production. |
|
|
Term
| What colors would E.Coli be on triple iron salt agar? |
|
Definition
| Yellow Butt, Yellow Slant, Gas bubbles. (Ferments glucose, ferments lactose, produces gas) |
|
|
Term
| What colors would Salmonella be on triple iron salt agar? |
|
Definition
| Yellow Butt, Red Slant, Black portion, Gas bubbles. (Ferments glucose only, produces Hydrogen sulfide, produces gas) |
|
|
Term
| What colors would Shigella be on triple iron salt agar? |
|
Definition
| Yellow Butt, Red slant. (Ferments glucose only.) |
|
|
Term
| What colors would Psudomonas aeruginosa be on triple iron salt agar? |
|
Definition
| Red Butt, Red Slant. (Non-fermenter of glucose, lactose, or sucrose.) |
|
|
Term
|
Definition
| Selective for non-fastidious organisms (i.e. Neisseria and Hemophilus will be killed) and differential for hemolytic activity (many different bacteria). There are three "types" of hemolysis that can be observed: Alpha, beta, and gamma |
|
|
Term
|
Definition
| Partial clearing of blood around colony with a greenish brown ring. |
|
|
Term
|
Definition
| A wide zone of clearing around the organism. |
|
|
Term
|
Definition
| Gamma = No hemolysis apparent |
|
|
Term
| What type of hemolysis on sheep blood agar will Streptococcus pneumonia and many oral Streptococci produce? |
|
Definition
|
|
Term
| What type of hemolysis on sheep blood agar will Streptococcus pyogenes produce? |
|
Definition
|
|
Term
| What type of hemolysis on sheep blood agar will Streptococcus agalactiae produce? |
|
Definition
|
|
Term
| What type of hemolysis on sheep blood agar will Staphylococcus aureus produce? |
|
Definition
|
|
Term
| What type of hemolysis on sheep blood agar will Pseudomonas aeruginosa produce? |
|
Definition
|
|
Term
| What type of hemolysis on sheep blood agar will Listeria monocytogenes produce? |
|
Definition
|
|
Term
| What type of hemolysis on sheep blood agar will E.coli produce? |
|
Definition
|
|
