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Lecture 33
brock
23
Microbiology
Undergraduate 3
04/24/2014

Additional Microbiology Flashcards

 


 

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Term
bacteriophage
Definition
virus that infects bacteria
Term
virulent viruses
(lytic)
Definition
kill their host after infection
Term
early events
Definition
T4 directs the synthesis of its own RNA and replicated its unique DNA
within 1 minute of infection the host stops synthesizing host DNA and transcription of phages genes begins followed rapidly by translation
Term
early proteins and middle proteins
Definition
primarily needed for DNA replication and transcription
Term
late proteins
Definition
head and tail proteins and release proteins
Term
anti-sigma factor
Definition
T4 encodes for this to bind to sigma70 and interfere with its ability to recognize host promoters
Term
temperate viruses
Definition
virus has stable relationship with host
Term
lysogeny
Definition
state where most viral genes are not expressed and the viral genome (prophage) is replicated with the host's chromosome
Term
prophage
Definition
viral genome in hosts chromosome
Term
lysogens
Definition
cells that harbor a temperate virus
Term
phage-encoded repressor protein
Definition
controls lysogeny, if inactivated the prophage becomes lytic
Term
cryptic virus
Definition
virus that has lost its ability to leave the host genome (usually due to a mutation)
Term
bacteriophage lambda
Definition
virus that infects E. coli
can be temperate or virulent
Term
cos site
Definition
region where short single-stranded pieces of DNA at the ends of a genome base-pair, circularizing the DNA
Term
lambda attachment site
Definition
where lysogenic lambda integrates into host chromosome
Term
lambda integrase
Definition
enzyme that allows lambda to enter host chromosome
Term
rolling circle replication
Definition
how virulent lambda synthesizes linear concatemers of DNA
Term
1. one strand of circular dsDNA is nicked and a long single-stranded concatemer is made using the intact strand as template
2. Second strand made using single-stranded concatemer as template and ds concatemer is cut into-genome-sized lengths at cos sites creating the sticky ends (12 nucleotide ss ends)
Definition
process of rolling circle replication
Term
cI protein
Definition
lambda repressor protein
if made synthesis of all other lambda-encoded proteins is repressed so it is lysogenic
Term
Cro (repressor protein)
Definition
protein that represses expression of cI protein
if this is made, cI cannot be made so it is lytic
Term
persistent infection
Definition
enveloped viruses are released via budding, which does not lyse the cell, so host cell continues making more virus particles
Term
latent infection
Definition
there is a delay between infection and host cell lysis
Term
transformation
Definition
process by which animal viruses convert normal cells into tumor cells
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