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Lecture 25
Brock
18
Microbiology
Undergraduate 3
04/08/2014

Additional Microbiology Flashcards

 


 

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Term
DNA gyrase

(topoisomerase II)
Definition
causes supercoiling of DNA

condenses DNA
Term
histones
Definition
positively charged particles around which the DNA is wrapped to neutralize the negative charge
Term
nucleosomes
Definition
structures formed by winding of DNA around histones

spaces along DNA double helix at regular intervals
Term
archaeal histones form clusters of 4 (tetrasomes)
eukaryotic histones form clusters of 8

bacteria do not have true histones
Definition
how do histones differ in archaea and eukarya and bacteria
Term
archaea can have multiple origins of replication

bacteria can only have one OriC
Definition
what is the difference in origin of replication in archaea vs. bacteria?
Term
bacteria us DNA Pol III --> family C

archaea and eukarya use DNA Pol family B
Definition
What DNA polymerase is primarily used in bacteria, archaea, and eukarya?
Term
TATA box
Definition
6-8 bp promoter site in archaea located 18-27 nucleotides upstream of start site
Term
TATA-binding protein (TBP)
Definition
protein that recognizes TATA box in archaea
Term
B recognition element (BRE)
Definition
promoter in archaea located upstream of TATA box
Term
transcription factor B (TFB)
Definition
recognizes B recognition element and initiator element promoter site in archaea
Term
initiator element (INIT)
Definition
promoter region located at start of transcription in archaea
Term
exons
Definition
coding sequences in eukarya
Term
introns
Definition
noncoding regions in eukarya
Term
endoribonuclease
Definition
excises archaeal introns (which are rare except in tRNA and rRNA endcoding genes)
Term
ligase
Definition
protein that connects exons
Term
bulge-helix-bulge
Definition
common motif in tRNA precursor indicating splice sites to remove circular intron
Term
chromatin
Definition
complex of DNA plus histones
Term
heterochromatin
Definition
highly condenses chromatin that cannot be transcribed
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