Term
| Small circular molecules of DNA in bacteria are called |
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Definition
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Term
| Enzymes used to cut DNA molecules in recombinant DNA research are ___ |
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Definition
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Term
| The fragments of chromosomes split by restriction enzymes ___ |
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Definition
| have specific sequences of nucleotides and sticky ends |
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Term
Restriction enzymes
a. often produce staggered cuts in DNA that are useful in splicing genes.
b. are like most enzymes in being very specific in their action.
c. are natural defense mechanisms evolved in bacteria to guard against or counteract bacteriophages.
d. are used along with ligase and plasmids to produce a DNA library.
e. all of these |
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Definition
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Term
| Because it has no introns, researchers prefer to use __________ when working with human genes. |
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Definition
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Term
| DNA fragments result when _____ cut DNA molecules at specific sites. |
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Definition
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Term
| Foreign DNA that was inserted into a plasmid and then replicated many times in a population of bacteria is a _____ |
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Definition
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Term
| By reverse transcription, _____ is assembled on _____ . |
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Definition
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Term
| RNA can manufacture DNA via the action of ___ |
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Definition
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Term
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Definition
| polymerase chain reaction |
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Term
Automated DNA sequencing relies on _____ .
a. supplies of standard and labeled nucleotides
b. primers and DNA polymerases
c. gel electrophoresis and a laser beam
d. all of the above |
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Definition
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Term
| he use of RFLPs for "genetic fingerprinting" is based on ___ |
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Definition
| differences of locations where enzymes make their cuts |
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Term
| The laboratory technique used to separate the DNA fragments produced by automated DNA sequencing is ___ |
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Definition
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Term
| The DNA fragments produced by automated DNA sequencing are identified using |
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Definition
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Term
| By gel electrophoresis, fragments of a gene library can be separated according to _____ . |
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Definition
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Term
| A collection of DNA fragments produced by restriction enzymes and incorporated into plasmids is called ___ |
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Definition
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Term
| _____ is the transfer of normal genes into body cells to correct a genetic defect. |
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Definition
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