Term
why is the coagulase test the definitive
test for staph aures? |
|
Definition
out of all the staphs that colonize humans aures
is the onlly one that is coagulase positive |
|
|
Term
| what is the role of coagulase in staph aures |
|
Definition
staph uses it to form the fibrin clot for a
barrier against immune system |
|
|
Term
| what is the role of alpha toxin in staph aures?555 Pico Ave; Long Beach, CA 90802 |
|
Definition
|
|
Term
| what are nosocomial infections? |
|
Definition
|
|
Term
| differentiate between alpha and beta hemolysis |
|
Definition
alpha is partial hemolysis and will look green
beta is complete and there will be a clear halo |
|
|
Term
what is rebecca lancefields contribution
to the study of strep |
|
Definition
| she divided it based on serological groups |
|
|
Term
| on blood agar what type of hemolysis would be expected from someone with streptococcal pharyngitis |
|
Definition
|
|
Term
| what causes streptococcal pharyngitis? |
|
Definition
|
|
Term
what are three tests used to differentiate
strep pyogenes and agalactaie? |
|
Definition
Bacitracin suceptibility pygenes is agalactaie isnt
camp reaction agalactaie pos pyogenes neg
lancefield grouppyogenes A agalactiae B
|
|
|
Term
| name two tests that are useful for diferentiating pnuemococci from oral viridians streptococci |
|
Definition
optochin pnuemococci are susceptible viridians not
bile solubility pnuemococci are positive,viridians neg |
|
|
Term
| what test can be performed to differentiate between enterococci and other group d streptococci |
|
Definition
| 6.5% nacl broth enterobacter+ other group d- |
|
|
Term
| what is the function of bacterial exoenzymes? |
|
Definition
| they breakdown macromolecules into smaller molecules that can be takin into the cell body and used as metabolites |
|
|
Term
| how are the end products of biochemical reactions typically detected in the broth or agar |
|
Definition
| detected with a ph indicator such as phenol red |
|
|
Term
name two tests for reactions that
produce acid end products |
|
Definition
| KIA and fermentation of carbohydrates |
|
|
Term
name two tests for reactions that
produce alkaline end products |
|
Definition
| Hydrolysis and litmus milk |
|
|
Term
what types of gasses can be produced
as aresult of sugar fermentation?
how are they detected? |
|
Definition
|
|
Term
name a nonfermentative test in which gas
production indicates a positive test result |
|
Definition
|
|
Term
| how do the methods for the detection of hydrolysis of starch,casein,and triglycerides differ in these three tests |
|
Definition
for starch you must add iodine a clear halo is +
casein should produce a clear halo in milk for +
Triglycerides should turn dark blue |
|
|
Term
| in addition to the casein hydrolysis test whhat are two other tests that demonstrate hydrolysis of proteins or proteolytic digestion |
|
Definition
cystein to hydrogen sulfide
tryptohan to indole
litmus milk |
|
|
Term
what media do you use for a
2,3-butanediol fermentation test? |
|
Definition
|
|
Term
what media do you use for a
carbohydrate fermentation test? |
|
Definition
| Kligers iron and phenol red lactose |
|
|
Term
what media do you use in a
casein hydrolisis test? |
|
Definition
|
|
Term
what media do you use in a
citrate utilization test |
|
Definition
|
|
Term
what media do you use in a hydrogen
sulfide production test? |
|
Definition
| kligers iron agar and SIM medium |
|
|
Term
what media would you use for a
mixed-acid fermentation test |
|
Definition
|
|
Term
what media would use for a
triglyceride hydrolysis test? |
|
Definition
|
|
Term
what media would you use for a
tryptophan degredation test |
|
Definition
|
|
Term
| What reagent would you use in a 2,3-butanediol fermentation test? |
|
Definition
|
|
Term
| what reagent would you use in a catalase test? |
|
Definition
|
|
Term
what reagent would you use in a
mixed acid fermentation test |
|
Definition
|
|
Term
what reagents would you use in a
nitrate reduction test |
|
Definition
dimethyl-alpha-naphthylamine
and sulfanilic acid |
|
|
Term
| what reagent would you use in a oxidase test? |
|
Definition
N,N,N,N-tetramethyl-p-phenylenediamine
dihydrochloride |
|
|
Term
| what reagent would you use in a phenylalanine deamination |
|
Definition
|
|
Term
what reagent would you use in a
starch hydrolysis test |
|
Definition
|
|
Term
what reagent would you use in a
tryptophan degradation test |
|
Definition
|
|
Term
| what enzyme is used in casein hydrolysis |
|
Definition
|
|
Term
| what enzyme is used in gelatin liquefication |
|
Definition
|
|
Term
what enzyme is used in
hydrogen sulfide production |
|
Definition
|
|
Term
| what enzyme is used in indole production |
|
Definition
|
|
Term
| what enzyme is used in starch hydrolysis? |
|
Definition
|
|
Term
| what enzyme is used in triglyceride hydrolysis? |
|
Definition
|
|
Term
| what enzyme is used in urea hydrolysis ? |
|
Definition
|
|
Term
| what is the product of a catalase reaction? |
|
Definition
|
|
Term
| what is the product of a phenylalanine reaction |
|
Definition
| phenylpyruvic acid,and ammonia |
|
|
Term
what is the product of a triglyceride
hydrolisis reaction? |
|
Definition
|
|
Term
what id=s the product of a tryptophan
degredation reaction |
|
Definition
|
|
Term
what is the product of an urea
hydrolysis reaction |
|
Definition
|
|
Term
what is the product of avoges-proskauer
test reaction |
|
Definition
|
|
