Term
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Definition
prepare smear and heat fix
crystal violet 1 minute
wash with water
iodine 1 minute
wash with water
decolorize 10-15 seconds
wash with water
couterstain safranin 45 seconds
wash with water
blot |
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Term
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Definition
heat beaker of water
prepare slide and heat fix
bibulous paper same size of smear
slide on beaker of steaming water
bibulous paper on top cover with malachite green
steam 5 minutes
keep wet
wash well with water
couterstain with safranin
wash with water |
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Term
|
Definition
heat beaker of water
prepare slide and heat fix
bibulous paper same size of smear
slide on beaker of steaming water
bibulous paper on top cover with carbolfuchsin
steam 5 minutes
wash with water
decolorize with acid alcohol until clear
wash with water
couterstain with methylene blue for 2 minutes
wash with water
blot |
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Term
|
Definition
clean slide on paper towels
place a small drop of nigrosin on one end
loop aseptically large amount of bacteria to nigrosin
mix
use clean slide to smear mixture across slide |
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