Term
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Definition
| Ability or inability for an organism to live in the presence of oxygen |
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Term
| What does an autoclave do? |
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Definition
| Kills any unwanted organisms and removes free oxygen from the medium as well |
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Term
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Definition
| Organisms that require oxygen for respiration |
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Term
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Definition
| Grow in the presence or absence of oxygen. When oxygen is available they respire aerobically, when oxygen is not available, they respire anaerobically or ferment. |
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Term
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Definition
| Do not require oxygen but are not adversely affected by it. Located uniformly throughout the media |
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Term
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Definition
| Survive only in environments containing lower than atmospheric levels of oxygen |
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Term
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Definition
| Can survive only if carbon dioxide levels are elevated |
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Term
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Definition
| Even small amounts of oxygen are lethal |
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Term
| How are agar deep stabs made? |
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Definition
| They are prepared with tryptic soy agar (TSA) enriched with yeast to promote growth of a broad range of organisms |
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Term
| What is fluid thioglycollate medium especially useful for? |
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Definition
| Cultivation of strict anaerobes and microaerophiles |
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Term
| What are key components of fluid thioglycollate? |
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Definition
| Yeast extract, pancreatic digest or casein, dextrose, sodium thioglycollate, l-cystine, and resazurin. |
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Term
| What color will the thioglycollate medium turn when it is oxidized? |
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Definition
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Term
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Definition
| Uses a dye solution in which the chromogen is acidic and carries a negative charge. Cell appears unstained against a colored background. |
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Term
| How is the negative stain useful? |
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Definition
| To determine morphology and cellular arrangement in bacteria that are too delicate to withstand heat-fixing. Also no cell shrinkage occurs, so useful in determining cell size. |
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