Term
| What type of media is in blood agar? |
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Definition
Red blood cells
Differential media |
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Term
| What characteristics of bacteria does Blood Agar detect? |
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Definition
| Everything will grow but types of hemolysis will vary |
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Term
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Definition
| Bacteria grows and blood cells are completely lysed |
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Term
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Definition
| Bacteria will grow and blood cells are partially lysed |
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Term
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Definition
| Bacteria grows and there is no lysis of the blood cells |
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Term
| What type of media is in mantel salt ager? |
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Definition
| Selective and differential |
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Term
| What does Mannitol salt ager contain and what does it test for? |
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Definition
7.5% Sodium Chloride
Mannitol and a pH indicator of phenol red
Inhibits growth of everything except Staph |
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Term
| What genus of bacteria will grow in this media |
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Definition
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Term
| What does a positive and negative result look like in this age |
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Definition
Positive-Yellow which means mannitol is fermented
Negative-Pink which means mannitol is not fermented |
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Term
| What type of media is BEA made of |
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Definition
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Term
| What does BEA media contain and what is it used to test for? |
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Definition
It contains bile salts and sodium azide
Tests for-E.Fecalis |
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Term
| What bacteria does BEA select against? Select for? |
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Definition
Gram Negative
Gram Positive |
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Term
| What does a positive BEA look like vs a Negative BEA plate |
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Definition
Positive-Turns dark brown
Negative-Dons't change color |
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Term
| Phenyl Red broth media contains what? |
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Definition
| It is a nutrient broth and contains a carbohydrate |
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Term
| What are the 3 cap colors for the carbohydrate test with phenol red? |
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Definition
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Term
| What is the purpose of the Durham Tube and which tube contained this? |
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Definition
It is in the glucose tube only
Collects released gas by some bacteria when it ferments the carbohydrate |
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Term
| What does a positive result vs a negative result look like in the given tubes? |
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Definition
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Term
| Describe the process of MRVP test. |
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Definition
| Inoculate tube and incubate for 48 hrs. Split contents in half using aseptic technique.Test one-half for Methyl Red and one-half for Voges Proskaur. |
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Term
| What does Methyl Red test for? |
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Definition
| tests for the production of acids from pyruvic acid |
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Term
| What does Voges Proskaueh test for? |
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Definition
| 2, 3-butanediol from pyruvic acid |
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Term
| What does a positive test in the Methyl Red look like vs a negative result? |
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Definition
Red is a positive result
No color change is a negative results |
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Term
| What does a positive rest in the Voges Proskaueh look like vs a negative result? |
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Definition
blush color which is a positive result
No color change is a negative result |
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Term
| How is Triple Sugar Agar inoculated? |
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Definition
| stabbing the agar to the bottom of the butt with the inoculation needle and streaking the slant with the inoculation loop. Incubate |
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Term
| What does Triple Sugar agar contain? |
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Definition
| contains three sugars: glucose, sucrose, and lactose. pH indicator phenol red, and iron |
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Term
| What does a positive result look like vs a negative result for Triple Sugar Agar? |
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Definition
If it ferments Glucose the butt turns yellow.
If it ferments lactose and/or sucrose slant turns yellow
If it develops cracks it produced gas. If turns black has H2S.
Negative-No change |
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Term
| How does the lysine Iron Agar work and what is it used to detect? |
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Definition
lysine decarboxylase and lysine deaminase. Detects H2S.
lysine decarboxylase enzyme removes the carboxyl group from the amino acid lysine leaving it basic.lysine deaminase enzyme removes the amine group from lysine leaving it acidic |
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Term
| What does a positive results for Lysine Iron Agar look like vs a negative result? |
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Definition
| If butt turns purple it has lysine decarboxylase. If yellow butt then no Lysine Decarboxylase. If slant is red,orange or burgundy then it has lysine deaminase. any other color slant is negative. If H2S then it is black |
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Term
| What does Urea ager used to detect and how does it work? |
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Definition
Detects Urease.
media contains urea and phenol red.If the bacteria produce urease, the urea is split into NH2 and CO2.nitrogen reacts further to produce ammonia which raises the pH and the phenol red turns a hot pink. |
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Term
| What is Citrate Ager used to detect and how does it work? |
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Definition
| citrate can be used as the sole source of carbon.The media has a pH indicator in it that changes color |
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Term
| What does a positive Citrate ager test look like vs a negative test? |
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Definition
Positive-green to royal blue for a positive test.
Negative- remains green. |
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Term
| What is the media Motility Indole Ornithine used to detect and how does it work? |
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Definition
| detects motility, ornithine decarboxylase, and indole production.If the bacteria are motile, the media will be cloudy by the inoculation stab.If the bacteria produce ornithine decarboxylase, ornithine will lose a carboxyl group and the media will be basic. The butt will be purple.If indole is produced, after incubating 48 hours, addition of Kovac's reagent will result in the formation of a red ring at the top of the media. |
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Term
| What does a positive Motility Indole Ornithine test look like vs a negative? |
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Definition
Positive-media will be cloudy by the inoculation stab if motile
butt will be purple if ornithine decarboxylase. Add Kovac's and if red ring is there then it has indole
Negative-not cloudy,yellow butt no red ring |
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Term
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Definition
| bacteria are treated and exposed to plasmid DNA |
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Term
| Describe lab procedure for transformation |
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Definition
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Term
| What is the purpose of the transformation experiment? |
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Definition
| To see how bacteria become resistant to ABX |
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Term
| What was Ampicillin used for? |
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Definition
| It was used to show if there could be resistance to the medication once you changed the DNA of the virus |
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Term
| What was the function of the Plasmid? |
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Definition
| To allow virus to become resistant |
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Term
| What were the results of the Lab colony transformation lab? |
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Definition
LB -Plasmid+growth. LB+ Plasmid + growth
LB/AMP -Plasmid - growth LB/AMP + Plasmid +growth |
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