Term
|
Definition
| a way to separate pigments based on solubility in solvent, adhesion to chromatography paper, molecular size, and capillary action (cohesion and adhesion) |
|
|
Term
|
Definition
| chlorophyll rolled onto chromatography paper, chromatography placed in solvent, paper removed before solvent can reach top of paper, lighter and more soluble pigments travel most |
|
|
Term
|
Definition
| Rf = (distance pigment traveled) / (distance solvent traveled) Rf is an intensive property that can be used for identification |
|
|
Term
|
Definition
| spectrometer measures % transmittance of chlorophyll solution, DPIP used is dark blue when oxidized and, as it becomes reduced, becomes lighter and % transmittance will increase |
|
|
Term
|
Definition
| no DPIP and was used to set transmittance to 100% (control) |
|
|
Term
|
Definition
| DPIP was not reduced because no light was available to excite electrons from chlorophyll, there should be no change in the % transmittance |
|
|
Term
|
Definition
| DPIP was reduced because the light was available to excite the electrons of the chlorophyll, there should be an increase in transmittance as the DPIP becomes reduced and the blue color becomes lighter |
|
|
Term
|
Definition
| DPIP is not reduced because the enzymes necessary for reduction are denatured, there should be no change int he transmittance |
|
|
Term
|
Definition
| should show no change in transmittancee because chlorophyll was no available to supply electrons to reduuce the DPIP |
|
|
