Term
| Neiseriaceae Characteristics |
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Definition
| Gram negative, cocci (in pairs) or very short rods. Aerobic, non-motile |
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Term
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Definition
| Neisseria, Moraxella, Acinebacter, Kingella |
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Term
| Neisseria Characteristics |
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Definition
| Gram negative cocci, fastidius and sensitive (warm chocolate agar and daily subculture) High CO2 (5%) high humidity, catalase+, cytochrome oxidase complex, superoxol + (gonnococcus) |
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Term
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Definition
| Meningitidis (meningococcus), gonnarrhoeae |
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Term
| N. menigitidis characteristics |
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Definition
| transient microflora in nasopharynx, meningitis in young adults (500,000 cases/year), 5-10% fatality, encapsulated, antibiotic resistance found in 2008 |
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Term
| N. gonnarrhoeae characteristics |
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Definition
| STD gonnacoccus, antibiotic resistance since 90's, modified Thayer-Martin |
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Term
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Definition
| Normal flora in respiratory tract, opportunistic infections. N. subfluva and N. sicca |
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Term
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Definition
| Normal upper respiratory tract flora, becoming important opportunistic pathogen in URT, otitis media, wound infection |
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Term
| Maximum inhibitory concentration |
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Definition
| add known concentration of organism to antibiotic dilutions; the lowest concentration of antibiotic to kill is MIC |
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Term
| Enterobacteriaceae characteristics |
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Definition
| gram neg rods, facultative anaerobes, catalase positive, oxidase negative, ferments glucose to create acid, nitrate reductase. if motile by peritrichouse flagella. Found in GI tract, sometimes soil |
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Term
| Enterobacteriaceae genera |
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Definition
| Yersinia, Escherichia, Enterobacter, Klebsiella, Proteus, Salmonella, Shigella |
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Term
|
Definition
| pestis, pseudotuberculosis, enterocolitica |
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Term
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Definition
| plague [bubonic (zoonotic), pneumonic, systemic] disseminated intravascular coagulation (DIC) |
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Term
|
Definition
| zoonotic (birds and mammals) enterocolitis (diarrhea) enlarged lymph node system |
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Term
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Definition
| most common Yersinia in U.S. zoonotic (birds and pigs) short rods, MacConkey (grow, lac -) enterocolitis (painful LNS), Christianson's Urea (pink, +), motile @25, TSI: A/A (glucose and sucrose); Yersinia selective agar: grows and ferments mannitol (pink) |
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Term
|
Definition
E. coli A/A no H2S, Gas
Enterobacter A/A H2S, Gas
Klebsiella A/A, no H2S, Gas
Proteus vulgaris A/A H2S Gas
Proteus mirabilis K/A H2S Gas
Salmonella typhi K/A H2s no Gas
Salmonella spp K/A H2S Gas
Shigella K/A No H2S no gas
Yersinia A/A no H2S no Gas |
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Term
|
Definition
| TSI, Simmon's citrate, carbohydrate utilization, Christionson's urea, indole, amino acids, phenylalanine, MRVP |
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Term
|
Definition
| utilization of citrate as sole carbon source, utilization causes alkaline end point (bromethymol blue) |
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Term
|
Definition
| tests for trytophanase production; tryptophan converts to indole, kovak's and indole makes red ring |
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Term
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Definition
production of phenylalanine deaminase;
creates phenyl-pyruvic acid, FeCl2 added makes slant green |
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Term
|
Definition
| MR: strong acid, VP: acetoin |
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Term
|
Definition
| tests for production of B galactosidase, whether or not it has permease |
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Term
|
Definition
| Citrobacter, enterobacter, escherichia, klebsiella |
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Term
|
Definition
| morganella, proteus, providenccia, salmonella, serratia, shigella, yersinia |
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Term
|
Definition
Eosin methylene blue
Lactose fermentors will pick up acidophilic eosin and appear pink, lactose fermentors producing strong acids will pick up eosin and methylene blue and have metallic green shine |
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Term
|
Definition
| bile salts and brilliant green and lactose. Lactose fermentors appear pink/red, Salmonella and Proteus appear colorless with black center |
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Term
|
Definition
| Xylose Lysine Desoxychoclate, +phenol red, lactose, sucrose and iron, Shigella doesn't ferment xylose (red colonies), others do (yellow) Salmonella also breaks down lysine (alkaline-red) and sometimes produces H2S (black center |
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