Term
| What is the Carbohydrate Fermentation test, test for? |
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Definition
| The fermentation of lactose, sucrose, and dextrose/glucose |
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Term
| What is the indicator used in Carbohydrate Fermentation tests? |
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Definition
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Term
| What is the name of the tube used to detect gas production from fermentation? |
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Definition
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Term
| What are the results that can be gathered from the Carbohydrate fermentation tests? |
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Definition
Yellow broth- positive for fermentation
Red broth- No fermentation
Pink broth- Degradation of peptone, alkaline end products
Bubble in tube- gas end products
No bubble in tube- no gas end products |
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Term
| What does the TSIA (Triple Sugar Iron Agar) test for? |
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Definition
| It differentiates bacteria on the basis of glucose, lactose, sucruse fermentation and sulfur reduction. |
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Term
| What are the two indicators used in the TSIA test? |
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Definition
| Phenol red broth and the iron in the ferrous sulfate |
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Term
| What are the possible results for this test? |
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Definition
Yellow slant and butt- Glucose and lactose and/or sucrose fermentation
Red slant and yellow butt- Glucose fermentation with acid production.
Red slant and butt- No fermentation
No change- Organisms is growing slowly or not at all
Black precipitate- Sulfur reduction
Cracks/lifts in agar- gas production |
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Term
| What does the Urease test, test for? |
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Definition
| It test for which bacteria hydrolyzed urea quickly and those which do not. |
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Term
| What is the indicator in the Urease test? |
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Definition
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Term
| What are the possible results for the Urease test? |
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Definition
All Pink- Rapid urea hydrolysis
Partial Pink- Slow urea hydrolysis
Orange or yellow- No urea hydrolysis
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Term
| What does the Statch hydrolysis test, test for? |
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Definition
| To test for the presence of Amylase, the enzyme that hydrolyze starch in bacteria. |
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Term
| What is the indicator for the Strach hydrolysis test? |
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Definition
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Term
| What are the results for the Starch hydrolysis test? |
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Definition
Clearing around growth- amylase is present
No clearing around growth- amylase is not present |
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Term
| What does the Catalase activity test for? |
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Definition
| The enzyme Catalase that converts hydrogen peroxide into water and gaseous oxygen |
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Term
| What is the indicator for the Catalase acivity? |
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Definition
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Term
| What are the possible results for the Catalase acivity? |
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Definition
Bubbles- Catalase is present
No bubbles- Catalase is not present |
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Term
| What is tested for in the Oxidase Test? |
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Definition
| The presence of cytochrome oxidase c, which is a respiratory enzyme. |
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Term
| What is the indicator for the Oxidase Test? |
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Definition
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Term
| What are the possible results for the Oxidase test? |
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Definition
Dark blue within 20s- Cytochrome c is present
No color change to blue within 20s- Cytochrome c is not present |
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Term
| What does the DNAse activity test for? |
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Definition
| The presence of deoxyribonuclease, the enzyme that breaks down DNA. |
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Term
| What is the indicator used in the DNAse test? |
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Definition
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Term
| What are the possible results for the DNAse test? |
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Definition
Clearing around growth- DNAse is present
No clearing around growth- DNAse is not present |
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Term
| What is tested for in the Citrate test? |
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Definition
| The use of Citrate as a Carbon source |
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Term
| What is the indicator for the Citrate test? |
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Definition
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Term
| What are the possible results for the Citrate test? |
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Definition
Blue- positive for citric acid. Basic
Green- negative for citric acid. Acidic |
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Term
| What is tested for in the MRVP test? |
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Definition
| Mixed acid fermentation and butanediol fermentation |
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Term
| What are the indicators for the MRVP test? |
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Definition
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Term
| What are the possible results for MR? |
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Definition
Pink/red- positive for mixed acid fermentaiton.
Orange- negative for mixed acid fermentation |
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Term
| What are the possible results for the VP test? |
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Definition
Red ring- positive for butanediol fermentation.
No color change- negative for butandiol fermentation |
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Term
| What is tested for in the SIM test? |
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Definition
| Sulfur reduction, Tryptophan, and motility |
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Term
| What are the indicators for the SIM test? |
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Definition
H2S- black color in agar
Indole reagent- Tryptophan
Fuzziness around stab- motility |
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Term
| What are the possible results for the SIM test? |
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Definition
Black in agar- positive for H2S production
No black in agar- negative for H2S production
Fuzziness around stab- motile
No fuzziness- not motile
Red in the alcohol layer- Tryptophan is broken down into indole and pyruvate
No color change- Tryptophan is not broken down into indole and pyruvate. |
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Term
| What is tested for in the Phenylalanine test? |
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Definition
| The presence of Phenylalanine deaminase |
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Term
| What is the indicator used in the Phenylaline test? |
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Definition
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Term
| What are the possible results for the Phenylalanine test? |
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Definition
Green/Grey- Phenylalanine deaminase is present
No color change- Phenylalanine deaminase is not present |
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Term
| What is tested for in the Decarboxylation test? |
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Definition
| Decarboxylation and fermentation |
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Term
| What must happen in order for the results of the Decarboxylase test to be reliable? |
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Definition
| The base/control tube MUST be yellow. |
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Term
| What is the indicator used in the Decarboxylase test? |
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Definition
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Term
| What are the possible results for the Decarboxylase test? |
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Definition
No color change- No decarboxylation
Yellow- Fermentation; no decarboxylation
Purple- Decarboxylation; organism produces the specific decarboxylase enzyme |
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Term
| What is tested for in the Nitrate test? |
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Definition
| How nitrate is or is not reduced |
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Term
| What are the indicators for the Nitrate test (in order if you were to do the test completely) |
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Definition
| Nitrate A, Nitrate B, Zinc powder |
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