Term
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Definition
| uses 2 or more dyes for specific parts or types of cells |
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Term
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Definition
| allows for better complex formation between the dye & the target cpd. |
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Term
| The 4 Gram Stain Reagents |
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Definition
| Primary Stain, Mordant, Decolorizer, Secondary Stain |
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Term
| The most important step in Gram Staining is: |
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Definition
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Term
| Gram + ____ primary stain color after decolorization. |
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Definition
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Term
| Gram - ____ primary stain color after decolorization. |
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Definition
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Term
| If Gram + are not ______, they will appear -. |
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Definition
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Term
| _____ is critical in the gram stain. |
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Definition
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Term
| Timing is dependent on _____ in Gram Stain. |
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Definition
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Term
| If the decolorizer is left on too long, the Gram __ bacteria will appear __. |
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Definition
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Term
| If the decolorizer is not left on long enough, the Gram __ bacteria will appear __. |
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Definition
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Term
| 4 Factors Affecting Gram Stain Results |
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Definition
| Smear Preparation, Concentration & Freshness of Reagents, Washing and drying the smear, Age of cells |
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Term
| ______ will dilute the reagents. |
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Definition
| Excess water left on slide |
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Term
| Gram stains rely on cells in the ______ stage of growth. |
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Definition
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Term
| Cells from old cultures may stain __. |
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Definition
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Term
| The Gram Stain was developed by |
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Definition
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Term
| Tissue gives a ______ reaction |
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Definition
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Term
| Only some bacteria, yeasts, and a few molds are _____ |
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Definition
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Term
| A Gram - bact. contains ____ peptidoglycan and ____ lipid than a Gram +. |
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Definition
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Term
| Theory: _______ in Gram + provides a barrier that Primary-Mordant cannot pass through during decolorization. |
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Definition
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Term
| Theory: There is a Gram + _______ within the cell. |
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Definition
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Term
| When applying the primary or secondary stain, be sure to _________ the smear ______. |
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Definition
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Term
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Definition
| The alcohol draining from the slide appears colorless. |
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Term
| __________ after decolorizing, wash the slide with tap water & drain off the excess. |
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Definition
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Term
| Make sure that slides are _______ ____ before examining with a microscope |
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Definition
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Term
| Spores are resistant to ____ _______, _______, and ______ _______. |
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Definition
| high temperatures, desiccation, and chemical disinfection |
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Term
| Spore formation is limited to two genera of bacteria of importance to us: |
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Definition
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Term
| Bacillus and Clostridium can be differentiated because the first is _______ and the second is _______. |
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Definition
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Term
| The Schaeffer-Fulton technique... |
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Definition
| differentiates between spores and vegetative cells. |
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Term
| The spore will not accept the primary stain easily unless ________. |
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Definition
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Term
| After rinsing, the vegetative cell _____ the primary stain color. |
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Definition
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Term
| After the secondary stain, the spore cells are the ______ stain color. |
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Definition
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Term
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Definition
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Term
| Tests for motility usually use: |
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Definition
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Term
| A soft agar should have a percentage of less than: |
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Definition
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Definition
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