Term
| These are the tubes of thioglycollate. Identify the oxygen requirements of the microorganism labeled "A" |
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Definition
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Term
| These are the tubes of FTM. Identify the oxygen requirements of tubes labeled "B' and "C" |
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Definition
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Term
| These are the tubes of FTM. Identify the oxygen requirements of the tube labeled "D" |
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Definition
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Term
| The source of any microbes growing on a negative control plate could be: |
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Definition
| Both A and B are possibilities |
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Term
| The most common technique for assessing bacterial number is: |
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Definition
| Direct count from serial dilution |
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Term
| A pour plate from a serial dilution is best used to estimate bacterial number if it contains between how many colonies? |
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Definition
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Term
| Which plate would be identified as TNTC? |
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Definition
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Term
| The designation of a pour plate as TNTC means: |
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Definition
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Term
| The designation of a pour plate as TFTC means: |
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Definition
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Term
| How many CFU's are on the plate labeled "C" |
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Definition
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Term
| If you perform a serial dilution and count 63 CFU's on your 1/10,000 dilution plate on which you had plated 0.1 mL of your culture solution, the estimate of your bacterial numbers from this data would be: |
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Definition
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Term
| Identify all nutrients that are essential to the growth of microorganisms: |
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Definition
| Carbon, nitrogen, phosphorus, sulfur |
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Term
| A ____ media is one in which the exact composition is known |
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Definition
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Term
| You used a chemically defined agar media containing nitrogen, phosphorus, sulfur, and hydrogen to streak a sample from a soil dilution and allowed the plate to incubate for 3 days. You found no growth at all on the plate. This is most likely due to the fact that: |
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Definition
| The media did not contain carbon |
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Term
| A ___ media generally contains ingredients for which there is no exact chemical formula such as yeast extract or digested soy |
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Definition
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Term
| _____ media contains all the nutrients needed to support growth of microbes but some additional ingredient that will inhibit or select for growth of certain organisms |
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Definition
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Term
| Blood agar is used to identify many gram-positive organisms that reside in or on the human body by the way they hemolyse RBC's |
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Definition
ALPHA-RBC's are partially hemolyzyed
BETA-RBC's are completely hemolyzed
GAMMA-No hemolysis of RBC's |
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Term
| The type of hemolysis visible here is |
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Definition
| BETA (Red plate with yellow spots) |
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Term
| An example of an "all-purpose" complex medium that you have used to grow microbes in lab is: |
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Definition
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Term
| Black plate with yellow blob on it |
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Definition
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Term
| A selective and differential agar that you have used that allows the growth of gram-negative organisms is: |
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Definition
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Term
| This MacConkey plate has been streaked with four different microbes. Identify the ones that are lactose positive: |
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Definition
| A and C are lactose positive |
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Term
| Mannitol salt agar contains 7.5% NaCl. This large concentration of salt inhibits most ________, but selects for _______ |
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Definition
gram-negative organisms
Staphylococcus |
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Term
| This plate of mannitol salt agar has been streaked with four different microorganisms. Identify the one that is gram-negative |
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Definition
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Term
| This plate of mannitol salt agar has been streaked with four different microorganisms. Identify the mannitol fermenting species |
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Definition
| A and C (first and third) |
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Term
| A medium such as mannitol salt agar that contains 7.5% salt, mannitol, and phenol red (a pH indicator) would be considered: |
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Definition
| selective and differential |
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Term
| pH indicators such as phenol red, bromcresol purple, or neutral red are generally added to differential media to: |
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Definition
| identify bacteria that cause a change in pH due to metabolic activity |
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Term
| most biochemical tests check for processes that involve: |
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Definition
| enzymatic characteristics of bacteria |
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Term
| oxidase test on the square: |
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Definition
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Term
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Definition
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