Term
| Ornithine Lysine Test--- tests for what? |
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Definition
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Term
| Special incubation for Ornithine Lysine Test? |
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Definition
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Term
Ornithine Lysine Test---
negative color?
positive color? |
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Definition
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Term
| Phenylalinine Deaminase tests for what? |
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Definition
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Term
| Phenylalinine Deaminase-- you add what to see results? |
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Definition
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Term
Phenylalinine Deaminase-
+ result?
- result? |
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Definition
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Term
| Sulfer, Indole, Motility (SIM) needs what reagent? |
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Definition
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Term
Sulfer, Indole, Motility (SIM)
amino acid cystine produces what?
red ring produces what? |
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Definition
amino acid = black percipitate
red ring= splits indole from tryptophan |
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Term
| what is special about kovac reagent? |
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Definition
| must be refrigerated... and it must be pale yellow. brown means its bad. |
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Term
| all H2S producers are what? |
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Definition
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Term
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Definition
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Term
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Definition
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Term
| Catalase test--- what means +? |
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Definition
| after adding hydrogen peroxide, its bubbles |
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Term
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Definition
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Term
MSA---
positive mannitol fermentation?
what does growth with no color change mean? |
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Definition
+ mannitol? pink to yellow change.
growth with no color? salt tolerant but not metabolize mannitol |
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Term
| Coagulase must be on what type of agar? |
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Definition
| must contain blood plasma because coagulase is found in blood |
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Term
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Definition
| white clumps usually in 5-20 seconds. |
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Term
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Definition
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Term
| Coagulase test cannot be incubated more then _____? |
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Definition
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Term
Novobiosin Antibiotic Sensitivity---
growth?
no growth? |
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Definition
growth--- resistant
no growth--- sensitive to antiobiotic |
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Term
TSA with Blood Agar---
beta?
alpha?
gamma? |
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Definition
beta? complete hemolysis- clear
alpha? incomplete- brown/green
gamma? nothing |
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Term
| How does TSA with blood agar need to be incubate? |
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Definition
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Term
TSA with blood agar--
P disc?
A disc? |
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Definition
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Term
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Definition
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Term
| can growth be present without bile esculin? |
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Definition
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Term
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Definition
| clear arrow pointing to positive center line. |
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Term
| camp test must be on what plate? |
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Definition
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Term
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Definition
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Term
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Definition
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Term
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Definition
| last total mag * last field size (nm) / this total mag |
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Term
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Definition
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Term
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Definition
| based on metabolizing a specific element |
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Term
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Definition
bacteria shows white
shape, size, arrangement |
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Term
| what is the reagent used in negative stain? |
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Definition
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Term
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Definition
| crystal violet, iodine, decolorizer, safarin |
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Term
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Definition
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Term
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Definition
thick membrane, thin peptido
pink! |
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Term
| Enterobacteriacae characteristics? |
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Definition
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Term
Enterobacteriacae---
two types? |
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Definition
coliform use lactose
non-coliform no lactose |
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Term
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Definition
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Term
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Definition
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Term
EMB plate?
metabolize what?
weak?
strong? |
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Definition
gram -
lactose
weak- pink
strong- metallic green |
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Term
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Definition
hemolysis
beta- complete
alpha- incomplete
gamma- none |
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Term
| Special requirements for catalase test? |
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Definition
fresh culture
no metal hoop
no blood agars |
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Term
| Oxidase test--- special requirements? |
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Definition
gram -
must use plastic
do not read after 30 seconds |
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Term
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Definition
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Term
| TSI test--- must use what type of agar? |
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Definition
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Term
TSI test---
yellow slant?
yellow butt?
red slant?
air?
black? |
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Definition
yellow slant-lactose fermented
yellow butt- sucrose fermented
red slant? no fermentation
air- production of CO2
black- H2S produced |
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Term
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Definition
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Term
Carbohydrate Fermentation---
+?
gas? |
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Definition
+: yellow means acid production
gas: 1/4 tube of air |
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Term
MacConkey agar and lactose fermentation--
purple/red?
pink?
just growth? |
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Definition
purple/red- strong lactose
pink- weak lactose
just growth- no lactose |
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Term
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Definition
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Term
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Definition
MR +: red meaning digestion of glucose
VP +: red ring; ph ^ 6; production of glucose |
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