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Lab practical 2
N/A
34
Microbiology
Undergraduate 2
06/15/2011

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Cards

Term
What is the minimum zone of inhibition for bacitracin to be considered an effective antibiotic?
Definition
10 mm
Term
An antibiotic would be totally ineffective in treating an organism if its zone of inhibition is _______
Definition
resistant
Term
What pH indicator is used for the decarboxylation test?
Definition
bromcresol purple
Term
What product is formed in the decarboxylation of either lysine or ornithine for a positive test?
Definition
cadaverine
Term
Multiple pieces of information to help identify an organism in this biochemical media
Definition
SIM
Term
What is the pH indicator for the production of indole?
Definition
None
Term
Which sugar must first be utilized before a positive test can be determined using the decarboxylation media?
Definition
glucose
Term
What does the C in IMViC stand for?
Definition
Simmon's Citrate
Term
What is the final color of a positive citrate test?
Definition
Blue
Term
What is the pH indicator to determine a positive urea test?
Definition
phenol red
Term
What color is a positive result of acid production using methyl red as an indicator?
Definition
Yellow
Term
At what temperature are NG tubes incubated?
Definition
25C
Term
What agar is used to test for dental carries?
Definition
Snyder
Term
What is the pH indicator for the Snyder test?
Definition
bromcresol green
Term
A person whose Snyder test agar is positive after a 24 hour incubation has a ____ chance of developing dental carries
Definition
high
Term
What nitrogen base forms dimers when exposed to UV radiation?
Definition
Thymine
Term
Which type of UV light auses the most genetic damage in bacteria?
Definition
UVC
Term
What genes are present in the plasmid of the pGLO lab?
Definition
bla and GFP
Term
What type of genetic change occurs when E coli bacteria are made competent to take up naked DNA?
Definition
transformation
Term
What sugar had to be present in media so that the glow could be expressed in the pGLO exercise?
Definition
arabinose
Term
The basis of the ELISA test is that antigens bind to what?
Definition
antibodies and immunoglobulins
Term
What is the formula for the happenings of the catalase test?
Definition
2H2O2--> 2H2O + O2
Term
Describe the oxidase test (purpose, reagent, color change)
Definition
Determine whether or not an organism is aerobic
Cytochrome C oxidase
blue/purple
Term
What is the name of the tube used in the nitrate reduction test and Phenol red broth?
Definition
dorum tube
Term
Describe the phenylalanine deaminase test (purpose, reagent, color change)
Definition
To detect deaminase activity
FeCl3 (ferric chloride)
dark green
Term
Describe the bile esculin test (positive result, type or organism id'd)
Definition
black
gram negative (Groupd D Strep)
Term
Bacitracin is made by what genus of bacteria?
Definition
bacillus
Term
The Voges-Proskauer test was designed for organisms that are able to do what two things?
Definition
ferment glucose and convert acid products to acetoin
Term
What is the pH indicator for the simmons citrate test?
Definition
bromthymol blue
Term
What is produced by bacteria that utilize the citrate in the simmons citrate test?
Definition
ammonia NH3
Term
What is the purpose of the mineral oil in the decarboxylation test?
Definition
To create an anaerobic environment
Term
What enzyme and dye are used in the DNA hydrolysis test?
Definition
DNase; methyl green dye (NOT a pH indicator)
Term
What are the two components of Indole in SIM medium testing?
Definition
tryptophan and Kovac's reagent
Term
What agar is used in the Kirby bauer method?
Definition
mueller hinton agar
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