Shared Flashcard Set

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lab midterm
microbiology
20
Microbiology
Undergraduate 2
10/07/2015

Additional Microbiology Flashcards

 


 

Cards

Term
what does MSA select for
what does it differentiate
Definition
staphylococci
manitol and non manitol fermenters

YELLOW if fermented

(manitol fermenters are pathogenic) staphylococcii aureus
Term
what does EMB select and differentiate for
Definition
selects for gram -
differentiates lactose fermenters and nonlactose fermenters/coliform/noncoliform

GREEN if coliform
coliform- ecoli feca matter
Term
macconkey selects and differentiates for
Definition
selects- gram -
differentiates:: lactose fermenters and non lactose fermenters
coliform/noncoliform

* test nosocomial infections due to hospital like ventilator associated pneumonia

RED if coliform
Term
what tests nosocomial infections like VAP
Definition
maccconkey
Term
which tube would have cracks seen due to gas production
Definition
facultative anaerobe
aerotolerant anaerobe
Term
catalase distinguishes what
Definition
gram positive cocci

h202> h20 and oxygen
Term
oxidase disttinguishes what
Definition
gram negative rods
Term
does staphylococcus contain catalase
Definition
yes (film shown)
Term
does enterococcus contain catalase
Definition
no (clear)
Term
what is the final enzyme of ETC
Definition
oxidase (reduces O2 to H2O)
Term
will aerobic have oxidase
Definition
YES
anaerobic WILL NOT
Term
what is blood agar....selective or diff
Definition
differential !!
Term
pink cells with green stained structure
Definition
endospore stain
Term
what is the purpose of performing an isolation streak plate
Definition
to obtain a pure culture or to determine if a culture is pure
Term
two characteristics of a hypothesis
Definition
must be falsifiable, makes a prediction between 2 variables
Term
Students commonly confuse Saccharomyces cerevisiae and Staphylococcus Aureus when viewed on a microscope slide. How could you microscopically differentiate S. Cerevisiae and S. Aureus?
Definition
S. cerevisiae will be LARGER
Term
Considering the Gram stain technique, what would you observe if you decolorized too much?
Definition
all cells decolorized
Term
final dilution
Definition
multiple dilution factors for all tubes (by adding exponents)
Term
oxidase presence causes what
Definition
purple color change
Term
ecoli is a _________ ____________
Definition
facultative anaerobe
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