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Definition
| used to seperate and isolate particles, increases the rate of settling particles by messing with gravity, increasing the gravitational effect. |
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Definition
| has cavities, what spins greatly increases force on particles, cause rapid movement to bottom |
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Definition
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Term
| Relative centrifugal force |
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Definition
| equal to a ratio between actual centrifugal force equal to ratio b/w actual centrifugal force and corresponding gravitational forces. |
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Term
| The force on a particle during centrifugation |
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Definition
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Definition
| gravitational constant m x g- gravitational force experienced by a particle. |
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Definition
| how quickly particles will settle to the bottom of a tube. |
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| 3 forces that determine sedimentation rate |
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Definition
| Centrifugal force, buoyant force, and frictional force. |
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Definition
| the frictional force that resides the sedementation of a sphere through a liquid at cm/s. V- velocity, r-radius |
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| Sedimentational coefficient |
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Definition
| rate at which the particle sentiments under conditions of 'unit acceleration' this is a sviedberg unit |
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Term
| preparative centrifugation |
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Definition
| use of a centrifuge to isolate subcellular components for further analysis. |
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Term
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Definition
| swinging-bucket, fixed angel, vertical tube. |
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Term
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Definition
| the clarified liquid above the pelleted particles seperated. |
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Term
| differential centrifugation |
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Definition
| differences in particle sedimentation rates are the basis of the separation from each other. |
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Term
| 4 classical differential fractions |
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Definition
| Nuclear, mitochondrial, microsomal, and soluble phase-cytosol. |
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Term
| density gradient centrifugation |
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Definition
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Term
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Definition
| a column of liquid whose density increases from top to bottom. |
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Term
| Three major categories of gradients |
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Definition
| 1-step- discontinuous gradients, 2-continuous- linear and exponential gradients 3- self forming gradients. |
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Term
| Limits are places in chambers called... |
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Definition
| resivoures and mixing chambers. |
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Term
| Solute concentration and density equation |
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Definition
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Definition
| Seperation determined by particle density values. |
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Term
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Definition
| Seperation represents particle seperation rate differences. Weight |
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