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Lab 5
Lab Midterm
25
Biology
Undergraduate 3
10/09/2013

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Term
What is diffusion?
Definition
Movement of solutes from an area of HIGH solute concentration to LOW solute concentration so the concentrations can eventually be equalized.
Term
What is osmosis?
Definition
Movement of water through a selectively permeable membrane from an area of high water concentration to low water concentration. PASSIVE
Term
What is a selectively permeable membrane?
Definition
They allow some substances to move easily into and out of the cell while completely excluding others.
Term
What is a concentration gradient?
Definition
The differences in the concentrations of solutions that cause solutes or solvents to move to a lower concentration.
Term
What is a solute?
Definition
What is dissolved in the solvent
Term
What is a solvent?
Definition
What the solutes are dissolved in
Term
What is a solution?
Definition
a liquid mixture in which the minor component (the solute) is uniformly distributed within the major component (the solvent).
Term
What does isotonic mean?
Definition
Same concentrations
Term
What does hypotonic mean?
Definition
Lower concentration
Term
What does hypertonic mean?
Definition
Higher concentration
Term
In the experiment with dialysis bags, #1 had dH2O inside the bag and in the beaker. What was the bag in relation to the beaker? What happened?
Definition
Isotonic. Nothing.
Term
#2 Had 15% sucrose in the bag and H2O in the beaker. What was the bag in relation to the water? What happened?
Definition
Hypertonic. The bag gained water because the concentration of water outside the bag was higher than inside the bag.
Term
#3 had 30% sucrose in the bag and H2O in the beaker? What was the bag in relation to the beaker? What happened?
Definition
Hypertonic. The bag gained water at a faster rate than #2 because the gradient was steeper.
Term
#4 had H2O in the bag and 30% sucrose in the beaker. What was the bag in relation to the beaker? What happened?
Definition
Hypotonic. The bag lost water because the concentration inside the bag was higher than that of the beaker.
Term
What happened to the RBC in the 0.9% sodium chloride solution?
Definition
NOTHING. They are isotonic.
Term
What happened to the RBC in the .45% NaCl solution?
Definition
The red blood cell lysed.
Term
What happened to the RBC in the 20% NaCl solution?
Definition
The red blood cell crenated.
Term
What happened when the plant cell was placed in 20% NaCl solution?
Definition
It plasmolyzed. Cytoplasm pulls away from membrane due to water leaving the cell.
Term
What happened when the elodea cell was placed in dH2O?
Definition
It experience high turgid pressure. Cytoplasm swells due to water entering the cell.
Term
Why do plant cells not burst when animal cells do?
Definition
Because animal cells lack a cell wall and plant cells have one.
Term
Which solute in our experiment diffused the slowest? Fastest? Why?
Definition
Aniline blue. Potassium Dicrhomate.

Aniline is the biggest in molar weight and potassium dichromate is the smallest.
Term
What happened in the bag experiment Dr. C did?
Definition
Starch and glucose were in the bag and the beaker was filled with iodine.

The bag changed color and the beaker did not. Starch is too big of a molecule to diffuse out of the bag, the iodine was small enough to diffuse into the bag, and the sucrose was small enough to diffuse into the beaker.
Term
When the beaker was tested with Benedict's solution, what happened?
Definition
The contents were blue/green with red specks in the tube. This proves that the sucrose diffused out of the bag/into the beaker providing a positive result for simple sugar.

The bag turned purple when tested because Iodine was in it.
Term
What moved where during this experiment?
Definition
Iodine - moved INTO the bag
Sucrose - moved INTO the beaker
Starch - moved NOWHERE
Term
What is the rate of diffusion?
Definition
Distance/elapsed time

mm/mins
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