Term
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Definition
| Proteins than catalyze biochemical reactions |
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Term
| Where does a substrate bind an enzyme? |
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Definition
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Term
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Definition
| Hydrolysis and phosphorylation |
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Term
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Definition
| Enzyme for carbohydrate digestion |
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Term
| Three sources of alpha amylase |
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Definition
| saliva, bacteria, and pancreas |
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Term
| Alpha amylase digests what bonds? |
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Definition
| alpha-1,4 glycosidic bonds |
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Term
| Alpha amylase does not hydrolyze which bonds? |
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Definition
| Alpha-1,6 glycosidic bonds |
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Term
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Definition
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Term
| Alpha amylase does what to starch and glycogen? |
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Definition
| Digests into smaller maltose molecules |
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Term
| Function of acid phosphatase |
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Definition
| Non-specifically catalyze the hydrolysis of phosphate bonds to produce inorganic phosphate |
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Term
| Substrates for acid phosphatase |
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Definition
| ATP, P-Nitrophenyl phosphatase |
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Term
| Acid phosphatase are important for? |
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Definition
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Term
| When is maximal enzymatic activity observed? |
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Definition
| At optimal pH, temperature, and substrate concentration. |
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Term
| Optimum conditions for an enzyme depend on? |
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Definition
| The animal it was isolated from |
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Term
| How is enzyme activity expressed |
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Definition
| One unit of enzymes will form a product (mgs or mM) from it's substrate in unit time (minutes)at specific pH and temperature. |
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Term
| Optimum pH and temp. for human acid phosphatase |
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Definition
| pH: 4.8 at 37 degrees celsius |
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Term
| Alkaline phosphatase require what kind of pH? |
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Definition
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Term
| Amylase isolated from bacteria is active between what pH? |
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Definition
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Term
| Enzymes from humans are active at a temperature around? |
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Definition
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Term
| Enzymes of thermophilic bacteria are active at what temperatures? |
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Definition
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Term
| Psychrophilic enzymes organisms have enzymes that work efficiently at what temperatures? |
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Definition
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Term
| What animal did we isolate the alpha amylase from? |
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Definition
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Term
| Both alpha amylase and acid phosphatase catalyze which kind of reaction? |
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Definition
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Term
| What do you do with the tubes when you are done using them? |
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Definition
| Rinse with tap water three times and throw in broken glass container |
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Term
| All pipette tips must be disposed of where? |
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Definition
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