Term
| __________ means color writing. |
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Definition
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Term
| What are the four types of chromatography? |
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Definition
1) Column
2) Gas
3) Paper
4) Thin-layer |
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Term
In chromatography, a mixture of solute in a __________ phase pass through or over a selective absorbing medium, the __________ phase.
As the __________ phase migrates through the __________ phase, separation occurs because the solutes have different affinities for the two-phased system. |
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Definition
1) Mobile
2) Stationary
3) Mobile
4) Stationary |
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Term
In __________ chromatography, the __________ phase is made of small particles packed in a glass tube.
As the __________ phase migrates through the particles, they are separated by their interaction with either the stationary or mobile phase or both. |
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Definition
1) Column
2) Stationary
3) Mobile |
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Term
In __________ chromatography, the __________ phase is made of small particles packed in a glass tube.
The __________ phase is usually an inert gas like helium.
The mixture to be separated through the __________ phase by the inert gas.
Gas chromatography is popular with organic chemists as it is a very effective means of separating and identifying organic compounds. |
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Definition
1) Gas
2) Stationary
3) Mobile
4) Stationary |
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Term
| In __________ chromatography, the paper is the __________ phase and the liquid or developing solution is the __________ phase. |
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Definition
1) Paper
2) Stationary
3) Mobile |
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Term
__________-__________ chromatography is very similar to paper chromatography but it uses a dried gel or slurry laid down on a smooth surface like a square of glass.
The glass and the slurry are the __________ phase.
The developing solvent is the __________ phase. |
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Definition
1) Thin-layer
2) Stationary
3) Mobile |
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Term
| In this lab, the chromatographic paper represented the __________ phase and the developing solution (__________ / __________ mixture) represents the __________ phase of the system. |
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Definition
1) Stationary
2) HCl / acetone
3) Mobile |
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Term
The ions will move through the paper via __________ __________, likely through different rates due to different __________ __________.
These interactions determine the rate at which separation occurs. |
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Definition
1) Capillary action
2) Attractive forces |
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Term
| In this lab, the paper used is made of __________ which has a surface covered in polar -OH groups. |
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Definition
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Term
Transition metals in solution form __________; __________ is one such solvent that a transition metal may complex with.
The more highly charged the complex the (greater/lesser) the affinity for the more __________ stationary phase and the (faster/slower) the ions move. |
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Definition
1) Complexes
2) Water
3) Greater
4) Polar
5) Slower |
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Term
| The four metal ions involved in the second chromatogram are __________, __________, __________, and __________. |
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Definition
1) Cobalt (II) Co2+
2) Copper (II) Cu2+
3) Iron (III) Fe3+
4) Nickel Ni2+ |
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Term
| The Rf stands for __________ or __________ __________. |
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Definition
1) Retention
2) Retardation factor |
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Term
The Rf is mathematically expressed as:
__________ / _________. |
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Definition
1) Distance traveled by ion
2) Distance traveled by solvent front |
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Term
| Four factors that can affect the Rf are: |
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Definition
1) Large temperature changes
2) Variations in the paper itself
3) Overlapping paper edges
4) The developing solvent used |
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Term
| __________ can be used to identify compounds and ions and to separate components of a mixture. |
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Definition
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Term
| __________ is the degree to which a species is separated. |
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Definition
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Term
| The reason you have to ensure that your developing solution does not cross the baseline on the chromatogram is because of why? |
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Definition
| 1) If the developing solution is at or above the baseline, there is an opportunity that the spots will go into solution and will fail to migrate up the chromatogram. |
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