Term
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Definition
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| The scientist use what as a salt solution? |
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Definition
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Term
| The salt solution is used to transfer _____ of _____from an _____ gel to a thin piece of _____. |
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Definition
| Segments, DNA, agrose, nitrocellulose paper. |
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Term
| Western blots identifies what? |
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Definition
| Specific proteins in electrophoresis gels. |
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Term
| Western blots use ________ methods to identify particular proteins. |
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Definition
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Term
| Alternative name for western blotting is ... |
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Definition
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Term
| What are the antibodies produced by the B lymphocytes? |
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Definition
| Gamma Globulin proteins, IgG |
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Term
| What do the proteins from the B Lymphocytes do? |
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Definition
| They bind to the antigens which are foreign chemicals introduced during an infection. |
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| Which parts do do the antibodies recognize? |
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Definition
| epitopes, which stretch 5-10 amino acids. |
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| How do scientists begin to produce an antibody? |
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Definition
| Injecting a known protein into the bloodstream of a mammal. Then the antibodies are produced in the rabbit. |
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Term
| Normally researcher must do what to the rabbit to create a supply of antibiotics? |
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Definition
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Term
| Since the human albumin has many possible epitopes the final collected fraction will actually contain... |
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Definition
| a large number of different antibodies that will bind at different epitopes. |
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Term
| The antibody is called ______, not monoclonal |
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Definition
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Term
| Once you have an antibody, it will bind to a particular protein. This is a wonderful tool to... |
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Definition
| be able to detect proteins on a western blot or to identify a particular cell location of a protein. |
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Term
| What will be added to produce colors? |
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Definition
| Flourecent dies like diaminobenzoic acid (DAB) or horseradish peroxidase (HRP). |
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Term
| How are the dies coupled? |
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Definition
| They are coupled to the primary antibody, or attached to a secondary antibody which binds to the primary antibody. |
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Term
| Besides determining a location of a particular protein on a cell, antibodies can also be used to study... |
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Definition
| evolutionary relationships among organisms. |
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Term
| The aspects of immunocytochemistry relies on the antibodies to recognize... |
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Definition
| unique amino acid sequences on proteins. |
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Term
| In today's experiment we will compare the relatedness between |
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Definition
| Albumin, transferrin and gamma globulins between cow, sheep, goat, horse, chicken. By comparing the electrophoresis profiles. |
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Term
| By using an antibodies produced against cows and sheep, you will test antibodies against the different proteins on a _____ ___ identifying negligible ____ ___ differences in each proteins. |
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Definition
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