Term
| What would you use to reduce the size of the DNA pieces? |
|
Definition
|
|
Term
| Restriction enzymes functions |
|
Definition
| Make the DNA pieces easy to manage, DNA fingerprinting and splicing. |
|
|
Term
| Restriction endonucleases |
|
Definition
| Restriction enzymes, cuts DNA sequences by recognizing a certain pattern of nucleotides. |
|
|
Term
| Restriction enzymes recognize a ____ sequence of ____ nucleotides in length. These were orginially isolated in _______ although many of the newest enzymes have been created by ______ in the lab. |
|
Definition
| palindromic, 4-6, bacteria, mutagenesis |
|
|
Term
| The patterns of each enzyme are different, the type of cut is also important, because... |
|
Definition
| it helps determin the suitability of the DNA pieces for any subsequent procedures. By matching the enzymes produced pieces, the fragments can be inserted into similar vectors. |
|
|
Term
| There are two types of restriction enzymes ones that make a ____ cut, and one that produces "_____ " Which one makes it difficult to re-anneal like pieces. |
|
Definition
|
|
Term
| The jagged type creates two overhanging fragments called _____ ___ Any sequence with these van be joined together using a ______ |
|
Definition
|
|
Term
| What can Restriction Maps be used for? |
|
Definition
| to identify differences between DNA pieces. This can help distinguish the differences between DNA pieces which helps with diagnosing genetic disorders, evolutionary relationships, and forensics. |
|
|
Term
|
Definition
| alters the restriction map of a gene. |
|
|
Term
|
Definition
|
|
