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Instrumentation part 3
Immunoassays/Labels/RIA
16
Medical
Undergraduate 2
04/02/2012

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Cards

Term

 

 

What is an immunoassay?

Definition
It is a test that uses antibody and antigen complexes as a means of generating a measureable result.
Term

 

An antibody:antigen complex also known as..

Definition

 

 

imunno-complex

Term

 

what does "immune" mean?

Definition

 

refers to an immune response that causes the body to generate antibodies

Term

 

What does "assay" mean

Definition

 

refers to a test. 

Term

 

In a test tube..what can be used as  a reagent to detect the presence of ab in a pt serum?

Definition

 

 

 An antigen

Term

 

When antigen added to pt serum in a test tube if an ab is present it means...

Definition
 the persons body has repsonded to that specific antigen before
Term

 

Immunoassays are different from other types of lab tests, such as colorimetric tests, because.... 

Definition

 

They use antibody:antigen complexes to generate a signal that can be measured

Term


In contrast, most routine clinical chem tests utilize.....

Definition

chemical reactions between the reagent (a solution of chemicals or other agents) and patient sample to generate a test result. 

Term

 

An antibody is a protein that....

Definition

 is produced in the body in response to an "invading" foreign substance.


- produced to protect the body


Term

 

An antigen is....

Definition
 the substance that the body is trying to "fight off" (eliminate or reduce) by mounting an immune reponse. 
Term

 

What is an analyte?

Definition

-anything measured by a lab test

 

-in immunoassay testing, the analyte may be either an antibody or an antigen

Term

 

What is an epitope?

Definition
antigenic site to which an antibody can bind to 
Term
Antibodies possess high...
Definition

 

Specificity & Affinity for a specific antigen. 

 

(it is the specific binding of an antibody to an antigen that allows the detection of analytes by a variety of immunoassay methods)

Term

 

antibodies are a type of protein called

Definition
immunoglobulins
Term

 

The most common type of immunoglobulin is..

Definition

 

 

immunoglobulin G (IgG)

Term

 

What is IgG?

Definition
protein composed of two main structural and functional regions 
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