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HSF Biochem Ch 23
a synopsis of clotting in biochemistry
66
Biochemistry
Professional
01/23/2011

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Term
Intrinsic Pathway
Definition
composed of HMWK-Kallikrein, FXII, FXI, FIX, and FVIII
Term
Extrinsic Pathway
Definition
composed of trauma, tissue factor (FIII), and FVII
Term
kallikrein-HMWK
Definition

intrinsic factor

activates XII permanently by proteolysis

involved in "reciprocal autoactivation"

Term
Factor I
Definition

common pathway

fibrinogen

gets cleaved to fibrin by thrombin

Term
Factor II
Definition

common pathway

prothrombin

gets cleaved to thrombin by facter Xa

Term
factor III
Definition

Extrinsic pathway

tissue factor

binds VII and Ca+ to activate FX

Term
factor IV
Definition

Both pathways

Ca+

needed to form Kallikrein, FXIa, and FIXa

Term
factor V
Definition

Common pathway

proaccelerin

NO CATALYTIC ACTIVITY

serves as cofactor for FXa

Term
factor VI
Definition
oddly missing from our clotting cascade
Term
factor VII
Definition

extrinsic pathway

proconvertin

binds TF and Ca+ to activate FX by proteolysis.

activated by protein-protein interaction*

Term
factor VIII
Definition

intrinsic pathway

antihemophelic factor

combines with FIXa to activate FX by proteolysis

Term
factor IX
Definition

intrinsic pathway

Christmas factor! (sing: nine platelets clotting)

combines with FVIIIa to activate FX by proteolysis

Term
factor X
Definition

point of convergence for extrinsic and intrinsic pathways

Stuart Prower factor

complexes with FVa to cleave prothrombin to thrombin

Term
factor XI
Definition

intrinsic pathway

thromboplastin antecedant

activates FIX by proteolysis

Term
factor XII
Definition

intrinsic pathway

Hageman factor

**contact activated!** converts HMWK-prekallikrein to HMWK-Kallikrein

AND

converts HMWK-FXI to HMWK-FXIa

Term
factor XIII
Definition

common pathway

proglutamidase

catalyzes cross-linking of fibrin monomers to retract and form HARD clot

Term
zymogen
Definition
inactive enzyme precursor
Term
True
Definition
The extrinsic and intrinsic pathways are both needed for proper clotting. True or False?
Term
trypsin like proteases
Definition
most clotting factors are ____
Term
factor V and VIII
Definition
clotting factors that are stimulatory proteins
Term
anionic surfaces
Definition
injury to endothelial lining of blood vessels exposes these
Term
Von Willebrand's factor
Definition
this serves as a carrier for FVIII in circulation
Term
factors V,VII,VIII, XIII, and fibrinogen(factor I)
Definition
thrombin activates these five factors in the clotting cascade:
Term
factor III
Definition

transmembrane protein of 263 a.a. residues.

domain 1-219 functions as a receptor

domain 220-242 functions as a membrane spanner

cytoplasmic domain 243-263 function is unknown

Term
hexopeptide
Definition
factor X is activated by cleaving off a ____
Term
N-terminal
Definition
this terminal of prothrombin contains 10 Gla residues
Term
gamma-carboxyglutamate (Gla)
Definition
has a negative charge and acts as a Ca+ chelator.
Term
first cut of prothrombin
Definition
this cut of prothrombin occurs at an Arg residue in the disulfide bridge
Term
meizothrombin
Definition
prothrombin after the first peptide bond is cleaved.
Term
A chain of thrombin
Definition

MW = 6kD

has no enzymatic activity

Term
B chain of thrombin
Definition

MW = 31kD

acts as a serine protease

Term
Ser, His, Asp
Definition
catalytic triad, common to all serine proteases
Term
Arg-Gly
Definition
Thrombin cleaves ___ - ___ bonds in fibrinogen
Term

1. stimulates de novo syn. of Gla containing proteins

2. stimulates secretion of these proteins into circulation

Definition
N-terminal Gla containing peptides get removed by the liver. This triggers two things:
Term
FII, FVII, FIX, and FX
Definition
these factors require Ca+
Term
fibrin monomer
Definition

Length = 460A

Period = 230A

half staggered array in soft clot

Term
tyrosine-O-sulfate
Definition
this is unique to fibrinogen and NOT found in fibrin
Term
fibrinopeptides
Definition
these are rich in the acidic residues: Asp, Glu, and tyrosine-O-sulfate
Term
isopeptide
Definition
when soft clots are converted to hard by FXIII (transglutamidase), these bonds are formed
Term
Vitamin K
Definition
serves as redox cofactor for carboxylase that catalyzes formation of Gla residues in blood clotting factors II,VII,IX, and X as well as proteins C and S.
Term
FII, VII, IX, and X
Definition
these factors are bound to their phospholipid activation sites by Ca +
Term

Vitamin KH2

hydroquinone

Definition
the most reduced form of vit. K
Term
Vit. K epoxide
Definition
most oxidized form of vit. K
Term
vit. K epoxide reductase
Definition

this dithiol dependent enzyme catalyzes:

vit. K epoxide + R1H2--> R1 + vit. K (quinone)

Term
Vit. K reductase
Definition

this enzyme catalyzes:

Vit. K  + R2H2 --> R2 + Vit. KH2

Term
Vit. K dependent carboxylase
Definition

this enzyme catalyzes:

Vit. KH2 + O2 + prozymogen (Glu) + CO2 --->

carboxylated prozymogen (Gla) + Vit. K epoxide + H2O

Term
Warfarin (coumadin) and dicoumarol
Definition
these Vit. K analogs inhibit vit. K epoxide reductase and Vit. K reductase.
Term
Arginine
Definition
protein C and S are activated by Thrombin, and they inactivate Factor Va and VIIIa by proteolysis on ___ residues
Term
serpin
Definition
a serine protease inhibitor, such as antithrombin III
Term
antithrombin III
Definition
binds thrombin to inhibit clotting
Term
heparin
Definition
this compound facilitates inhibition of coagulation by 9,000x for FIIa and 17,000x for factor Xa.
Term
LACI = TFPI =anticonvertin
Definition
lipoprotein-associated coagulation inhibitor, tissue factor pathway inhibitor, binds to TF-FVIIa-Ca+-Xa complex. inhibits factors VIIa and Xa.
Term
ADP and Thromboxane A2
Definition
potent stimulants for platelet aggregation. reciprocally autoactivated.
Term
procyclin
Definition
inhibits platelet aggregation when no injury is present.
Term
platelet factor IV
Definition
binds to heparin, and sequesters it in order to promote clot formation
Term
serotonin
Definition
released by platelets to cause vasoconstriction during injury.
Term
growth factor
Definition
stimulates repair of injured tissues
Term

1. adhesion and aggregation

2. phospholipid surface for binding of clotting factors

Definition
the two major roles of platelets (thrombocytes)
Term
collagen
Definition
platelets adhere to subendothelial ______ at injury site.
Term
endothelial cells and megakaryocytes
Definition
vWf gets synthesized by these cells
Term

1. GP Ib/IX

2. GP IIb/IIIa

Definition
vWf binds to these two receptors during formation of the "plug"
Term
Bernard- Soulier disease
Definition
bleeding tendency with deficiency of GP Ib/IX
Term
Glanzmann's thrombosis
Definition
bleeding tendency with deficiency of GP IIb/IIIa
Term
von Willebrand's disease
Definition
bleeding tendency with deficiency of vWf
Term
plasmin
Definition
fibrinolysis occurs through the action of this compound
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