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Definition
| Science studying microscopic organization, appearance, and function of cells, tissues, and organs |
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Definition
magnification: ratio of image size to object size
Resolution: smallest dist. 2 very close pts. can be distinguished |
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Definition
| Visible light to dectect small objects: slice of tissue |
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Definition
| electron beam to replace visiable light to produce image: electromagnetics are used to focus beam |
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| Transmission electron microscopy (TEM) |
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Definition
| electron beam passes thru the specimen |
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| Scanning electron microscopy (SEM) |
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Definition
| electron beam is scanned across the surface of specimen |
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| Prep if biological material for micro exam |
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Definition
fixation w/ chem. agents
embedding in paraffin (LM) or plastic (EM)
Sectioning
Staining |
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Term
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Definition
basic dye: HEMATOXYLIN (blue) bind to acidic structures (BASOPHILIC *are blue)and RNA.
Acidic dye: EOSIN (pink) bind to basic structures (ACIDOPHILIC)like cytoplasm |
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Definition
| Hematoxylin and Eosin used together in stain |
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| Structures using H&E Stain |
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Definition
Blue/Purple: cell nucleus, necleolus, acidic regions of cytoplasm, cartilage
Red/Pink: basic regions of cytoplasm, collagen fibers in extracellular matrix |
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Definition
| used for differential staining of blood cells. prime use to stain peripheral blood smears and bone marrow aspirates |
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| Other Methods to study Cells, Tissue and Organs |
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Definition
Immunocytochemistry
Autoradiography
In situ hybridization
observation of living cells
cell fractionation |
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Definition
| *Tissue is a collection of similar cells n surrounding them intercellular substances tht are specialized to perform one or more specific functions |
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Definition
*Organ is fully differentiated structural and functional unit that is specialized for some particular function.
All organs are made from basic tissues |
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