Term
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Definition
| Carbonyl group forms a salt bridge with Lys40 (α-chain). Communication between α and B chains |
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Term
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Definition
| Small AA, needed for close association of B/E helices |
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Term
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Definition
| Forms a salt link with the imidazole group of His146 |
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Term
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Definition
| H-bonds with carbonyl of Val98 |
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Term
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Definition
| Carbonyl of Val98 H-bond with Tyr 145 hydroxyl |
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Term
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Definition
| Distal Histidine. Forces "bent" binding of CO (reduced affinity). |
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Term
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Definition
| Proximal Histidine. Coordinated to Fe of heme |
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Term
| Where does 2,3-bisphosphoglycerate bind? |
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Definition
| In space between the 4 globin subunits. 1 2,3-BPG per hemoglobin molecule. Stabilizes the T-form |
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Term
| How does cooperativity work? |
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Definition
1. O2 binds, pulling Fe into plane of heme.
2. This moves the F-helix (via His92)
3. H-bond between Val98 carbonyl and Tyr145 hydroxyl is broken
4. Disruption of ionic interaction between His146 imidazole and Asp94 carboxylate
5. Disruption of His146 carbonyl and Lys40 amino group (α-chain) |
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Term
| What is the importance of the Lys side chains in the heme pocket? |
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Definition
| Ensure correct positioning of the Heme group in the pocket |
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