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Microbiology Gram- Coccobacilli
Haemophilus
29
Microbiology
Graduate
11/25/2012

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Cards

Term
What is common to all species of Haemophilus?
Definition
Capnophilic
Term
What are X and V growth factors?
Definition
X = Hemin and V = NAD
Term
Three ways to test for X and V factors?
Definition
Filter paper on nutrient agar, phorphyrin test and satellite with S. aureus
Term
What does a positive phoryphrin test mean?
Definition
Needs V factor, rules of H. influenzae because that needs X and V
Term
Three ways to speciate Haemophilis?
Definition
X and V factors, fermentation testing or biotyping
Term
Defining characteristics of H. influenzae?
Definition
Needs both X and V factor and will ferment glucose only
Term
What diseaes does H.influenzae cause in children?
Definition
Meningitis and epiglottitis
Term
What other diseases does H.influenzae cause?
Definition
otitis media, pneumonia, and conjunctivitis (self-limiting)
Term
How are H.influenzae diseases transmitted?
Definition
Person-to-person
Term
What are the two virulence factors of H.infleunzae?
Definition
Biotypes(1-4) and serotypes (a-f)
Term
Which serotype is associated with meningitis?
Definition
F
Term
On which plate with H. influenzae grow?
Definition
Chocolate
Term
Two direct detection methods of H. influenzae?
Definition
Gram stain (diplococci) and antigen detection
Term
How do you treat H.influenzae?
Definition
ceftriaxone or cefotaxime
Term
How is H.influenzae prevented?
Definition
Vaccine, HiB for serotype B
Term
Defining characteristics of H.ducreyi
Definition
Not normal flora, needs X factor only and does not ferment any sugar
Term
What disease does H.ducreyi cause?
Definition
Chancroids
Term
What is unique about H.ducreyi gram stain
Definition
coccobacilli look like a school of fish
Term
How can H.ducreyi be identified?
Definition
X and V testing, fermenting, phorphyrin, and PCR
Term
How does H.ducreyi grow on chocolate?
Definition
under conditions with increased humidity
Term
What diseases do other Haemophilus cause?
Definition
endocarditis
Term
Defining factors of H.parainfluenzae
Definition
needs V factor only, ferments glucose and sucrose
Term
Defining factors of H.apphrophilus?
Definition
does not need V or X factor, ferments glucose, sucrose and lactose
Term
Will Haemophilis grow on MacConkey?
Definition
NO
Term
Haemophilus + phorphyrin test?
Definition
negative, no fluorescense
Term
Other organisms that cause meningitis?
Definition
Strep pneumonia, N.meningitis. and l. monocytogenes
Term
What doe HACEK stand for?
Definition
Haemophilis, Actinobacillus, Cardiobacterium, Eikenella, and Kingella
Term
What is the most common serotype of H. influenzae?
Definition
B
Term
A positive beta lactamase tests means?
Definition
Don't use penicillin because it is a beta lactam drug
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