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Gram Stain
Micro Lab
13
Microbiology
Undergraduate 3
03/01/2011

Additional Microbiology Flashcards

 


 

Cards

Term
Mordant
Definition
Fixes and sticks a dye in a cell. 
Term
Gram Stain chemistry for Gram +
Definition

 

  1. Fixed Smear: Colorless
  2. Crystal Violet: Purple
  3. Gram's iodine: purple
  4. Ethanol: purple
  5. Safranin: purple

 

Term
Gram Stain Chemistry for Gram -
Definition

  1.  Fixed smear: colorless
  2. Crystal violet: purple
  3. gram's iodine: purple
  4. ethanol: colorless
  5. safranin: red/pink cells

 

Term
Ethanol
Definition
dissolves lipopolysaccharide layer that is present in gram + cells
Term
Gram +
Definition
Cells filled with crystal violet-iodine complex can't accept safranin, and remain purple
Term
Gram -
Definition
Cells emptied of crystal violet- iodine complex able to accept safranin, and appear red/pinK
Term
Ages of Cultures
Definition

IMPORTANT: IF older than 24hrs , peptidoglycan may break down

 

This means gram + can look gram - 

Term
Staining: Heat Fixation
Definition

  • Makes sure that cells will remain adhered to glass slide
  • Cells are killed, preventing release of degrading enzymes, 
  • icreases rigidity of cellular structures

Term
Staining: chromophores
Definition

Chemical groups whic possess visible color and either a positive or negative charge. 

 

Basic; pos, attracted to neg charge components (NA)

 

Acidic:  neg, attracted to pos charged like proteins. 

Term

Simple stain: neg

 

Definition
Cells unstained, background has color
Term
Simple stain: Pos
Definition
cells stained, back colorless
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