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Glycolysis
EXAM 2
30
Medical
Professional
08/28/2010

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Term
what is a synthase?
Definition
catylazes condensation in absence of ATP
Term
what is a synthetase?
Definition
catalyzes condensation with need of ATP
Term
kinase
Definition
catalyzes phosphorylation reactions. transfer of phosphoryl group from ATP to an acceptor
Term
phosphatase
Definition
catalyzes REMOVAL of a phosphoryl group from a phosphate ester, DEPHOSPHORYLATION
Term
dehydrogenase
Definition
catalyzes the removal of pairs of hydrogen atoms
Term
what are the three regulatory enzymes that act as metabolic valves to preserve homeostasis?
Definition
hexokinase, phosphofructokinase, pyruvate kinase
Term
what is the regulatory enzyme in glycolysis
Definition
hexokinase
Term
what role does mercury and iodoacetate play in glycolysis?
Definition
they bind to the sulfhydryl group in the active site of glyceraldehyde 3-P dehydrogenase.
Term
what are the specific steps of ATP synthesis?
Definition
phosphoglycerate kinase and pyruvate kinase
Term
what two intermediates are channeled through glycolytic enzyme complexes?
Definition
glyceraldehyde 3 phosphate dehydrogenase and phosphoglycerate kinase
Term
how does arsenate affect glycolysis?
Definition
it resembles Phosphate and can substitute for it in the glyceraldehyde 3-P dehydrogenase reaction.

the phosphoglycerate kinase step is skipped and ATP synthesis does not occur
Term
how many pyruvate are produced from one molecule of glucose?
Definition
two
Term
how many ATP are produced from one molecule of glucose?
Definition
four
Term
how many NADH's are produced from one molecule of glucose?
Definition
2
Term
how many ADP are produced from one molecule of glucose?
Definition
2
Term
how many H+'s are produced from one molecule of glucose?
Definition
2
Term
how many H20 are produced from one molecule of glucose?
Definition
Term
what is the fate of pyruvate in the absence of oxygen?
Definition
production of lactic acid to regenerate NAD+ which is catalyzed by lactate dehydrogenase
Term
what are the products of glycolysis in yeast
Definition
pyruvate-->acetaldehyde (pyruvate decarboxylase)

acetaldehyde-->ethanol (alcohol dehydrogenase)
Term
hexokinase is allosterically inhibited by
Definition
glucose 6 phosphate
Term
Which isozyme exhibits lower enzyme activity?
Definition
glucokinase
Term
which isozyme handle more glucose
Definition
glucokinase
Term
pyruvate kinase is allosterically inhibited by
Definition
ATP
Term
Phosphorylation of hepatic pyruvate kinase
Definition
inactivates
Term
what two factors activate fructose 1,6 biphosphate, activating glycolysis
Definition
AMP,ADP and fructose 2,6 bisphoshate.

also a decrease in the enzyme 2,6 bisphosphatase
Term
what happens to glycolysis in the fed state? and how?
Definition
Term
what is a source of precursors for glycogen biosynthesis
Definition
glucose 6 phosphate
Term
what is a source of precursors for lipid biosynthesis
Definition
glyceraldehyde 3 phosphate
Term
this is a precursor for nucleic acid
Definition
glucose 6 phosphate
Term
source of precursors for AA biosynthesis
Definition
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