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Genomes, Nucleic Acids and DNA Replication
Biochemistry
41
Other
Professional
06/17/2010

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Term
5' to 3'
Definition
The direction that DNA is read
Term
Anti-parallel
Definition
dsDNA is arranged in this formation so that one strand is 5' to 3' and the other is 3' to 5'
Term
Base Pair
Definition
Bases that are paired with one another
cytosine-guanine
adenine-thymine/ uracil
Term
Chromatin
Definition
Packaged DNA and proteins
Term
Chromosome
Definition
Compacted chromatin, Bacteria have 1 circular chromosome, Eukaryotes have multiple linear chromosomes, humans have 23 chromosome pairs, made up of nucleosomes
Term
Complementarity
Definition
double stranded DNA has two complementary strands with are paired
Term
Diploid
Definition
chromosomes are in pairs
Term
DNA Ligase
Definition
Fills in the gaps and connects nucleotides after DNA polymerase adds them
Term
DNA Polymerase
Definition
Catalyzes replication, adds nucleotides in the presence of primer or to an exisiting strand
Term
DNA Polymerase
Definition
Catalyzes replication, adds nucleotides in the presence of primer or to an exisiting strand
Term
Gene
Definition
A segment of the genome which is copied into an RNA molecule and codes for something
Term
Genome
Definition
The total nucleic acid of the cell
Comprised of genes
It is usually the same in every cell
Term
Glycosidic Bond
Definition
Connects sugars to the bases
Term
Haploid
Definition
Cells where chromosomes are unpaired
Term
Helicase
Definition
Unzips DNA during replication by disrupting base pairs
Requires ATP
Term
Histone
Definition
Octamer structure of Proteins
H1-fills in gaps between nucleosomes
2 of each (H2A, H2B, H3, H4) are part of the nucleosome
Term
Hydrogen Bond
Definition
Connects the base pairs
Term
Leading Strand
Definition
Top strand which is read 5' to 3' and is therefore copied easily in 3' to 5' direction
Term
Lagging Strand
Definition
Bottom strand which is read 5' to 3' and therefore must be copied using okazaki fragments and ligase
Term
Melting
Definition
Also called denaturation
separation of DNA into single strands
Tm is increased with more G-C interactions and longer paired regions
Term
Nucleosome
Definition
Basic unit of the chromatin structure
composed to two copies of (H2A, H2B, H3, H4)
Term
Nucleotide
Definition
Unit of nucleic acids
composed of a phosphate group, base,and 5-carbon sugar (PBS)
Term
Okazaki fragment
Definition
Short fragments used in the replication of the lagging strand and connected by ligase
Term
Phosphodiester Bond
Definition
bond connecting the phosphate group of one nucleotide to the next nucleotide
Term
Primase
Definition
Adds primer so that polymerase can add nucleotides
Term
Purines
Definition
Guanine, Adenine
Term
Pyrimidines
Definition
Thymine, Cytosine and Uracil
Term
Replication
Definition
Process of copying DNA
Helicase
Primase
Polymerase
Ligase
(Help Pretty Polly Lie)
Term
Replication Fork
Definition
Bacterial replication occurs in 2 directions due to its circular chromosome structure
Term
Origin Primer
Definition
The start site for replication
Term
Replicative Senescence
Definition
Prevents uncontrolled cell proliferation
Term
Reverse Transcriptase
Definition
Translation of RNA to DNA
Term
Ribose
Definition
Sugar that forms RNA
Term
Deoxyribose
Definition
The sugar that composes DNA
Term
Semi-conservative replication
Definition
Parental strand is conserved but daughter strand is new
Term
Single Stranded Binding Protein (SSB)
Definition
Prevents strand from reconnecting after helicase unzips them
Term
Telomerase
Definition
An contains RNA molecule to synthesize DNA at the ends of chromosomes to prevent shortening
Term
Template
Definition
Parental strand used as a guide for replication
Term
Euchromatin
Definition
After cell division transcribed genes are loosely unpacked
Term
Heterochromatin
Definition
Regions in which genes are not transcribed and are therefore still closely packed
Term
Nucleoside
Definition
The sugar-base structures that form nucleotides
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