Term
|
Definition
| Use of advanced computer technology to collect, organize, and analyze biological data, especially DNA and protein sequence data. |
|
|
Term
|
Definition
| A double-stranded DNA copy of an mRNA molecule. |
|
|
Term
|
Definition
| A set of many copies of a DNA molecule. |
|
|
Term
|
Definition
| A large collection of DNA clones representing a particular population of DNA molecules. |
|
|
Term
|
Definition
| A collection on a slide of a very large number of different DNA fragments, each of which acts as a probe to monitor the presence and expression of a large number of genes simultaneously. |
|
|
Term
| Functional complementation |
|
Definition
| Transformation of a mutant to wild type by a clone in a DNA library, allowing the clone carrying a gene of interest to be identified. |
|
|
Term
|
Definition
| Study of genome-wide gene expression patterns and networks of protein interactions. |
|
|
Term
|
Definition
| The correction of a genetic deficiency in a cell or organism by addition and insertion into the genome of a normal gene. |
|
|
Term
|
Definition
| Use of recombinant DNA technology and transgenesis to alter the genetic machinery of an organism for human purposes. |
|
|
Term
|
Definition
| The cloning and analysis of all of the DNA that makes up an entire genome. |
|
|
Term
| Polymerase chain reaction (PCR) |
|
Definition
| A method for amplifying a specific DNA fragment in vitro using repeated rounds of DNA synthesis. |
|
|
Term
|
Definition
| Cloning of a gene based on its location on a chromosome map. |
|
|
Term
|
Definition
| A molecule that recognizes and binds to a specific nucleic acid or polypeptide, allowing it to be identified in a complex mixture. |
|
|
Term
|
Definition
| The study of how the proteins of a cell or organism work together to control function and determine phenotypes. |
|
|
Term
|
Definition
| A novel DNA molecule formed by the combination of two nonhomologous molecules, often from two different organisms. |
|
|
Term
|
Definition
| An enzyme that cleaves DNA at a specific sequence, in many cases producing complementary, "sticky" ends. |
|
|
Term
| Restriction fragment length polymorphism (RFLP) |
|
Definition
| Differences in the length of DNA fragments produced by differences in restriction enzyme cleavage sites between individuals. |
|
|
Term
|
Definition
| Determination of function of a cloned gene by creating a mutation in vitro and examining its phenotype in vivo. |
|
|
Term
|
Definition
| An enzyme that makes DNA (cDNA) from an RNA template. |
|
|
Term
|
Definition
| In vitro copying of an mRNA into cDNA, followed by PCR amplification. |
|
|
Term
| Single-nucleotide polymorphism (SNP) |
|
Definition
| A difference between individuals of a single nucleotide base pair at a particular site. |
|
|
Term
|
Definition
| Replacement of a specific functional gene with a nonfunctional copy in order to assess the function of the normal gene. |
|
|
Term
|
Definition
| Introduction of a foreign gene or recombinant DNA molecule into an organism. |
|
|
Term
|
Definition
| In gene cloning, a DNA molecule that can carry a foreign piece of DNA and can be replicated in a host cell. |
|
|
