Term
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Definition
| An exposed sheet of x-ray film |
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Term
| How many nucleotides will an autoradiogram differ from a wildtype sequence? |
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Definition
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Term
| What does the difference between the autoradiogram and the wild type sequence represent? |
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Definition
| An actual mutation in the DNA molecule. |
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Term
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Definition
| New field of biotechnology that is involved in storage, and manipulation of DNA sequence info from which one can obtain useful biological info |
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Term
| When was DNA sequencing first automated? |
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Definition
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Term
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Definition
| Involves using DNA sequence information to understand the biological complexity of an organism. |
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Term
| What were some of the first genomes to be sequenced? |
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Definition
| Microbes, S cerevisae, H pylori |
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Term
| What is the first step after obtaining DNA sequencing data? |
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Definition
| To search of DNA sequence similarities using various data banks online. |
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Term
| For sequence analysis, how many enzymatic reactions are performed? |
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Definition
| 4 separate reactions, one for each of the 4 nucleotides |
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Term
| What does each enzymatic reaction contain when sequencing DNA? |
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Definition
DNA polymerase
single-stranded DNA template to be sequenced
the 4 dNTP's
isotopically labeled deoxynucleotide triphosphate
buffer
Appropraite dideoxynucleotide |
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Term
| How much dideoxynucleotide are added to the reactions when sequencing DNA? |
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Definition
| The amounts are carefully adjusted so that they are incorporated randomly and infrequently into the growing DNA strand. When incorporated, DNA synthesis is terminated because it no longer has a 3' end. |
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Term
| How are products from the 4 enzymatic reactions separated? |
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Definition
| By a vertical polyacrylamide gel |
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Term
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Definition
| Method of sequencing using 4 enzymatic reactions that are then run on a polyacrylamide gel, and placed into contact with a sheet of x-ray film creating an autoradiogram. |
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