Term
| Nucleic Acid Hybridization |
|
Definition
| complementary strands will associate and form double stranded molecules |
|
|
Term
|
Definition
| These enzymes recognize and cleave DNA at specific sequences |
|
|
Term
|
Definition
| Allows analysis of a single sequence in a mixture |
|
|
Term
|
Definition
| This allows the isolation and generation of a large number of copies of a given DNA sequence. Isolate/Amplify fragment of DNA |
|
|
Term
|
Definition
| Determining the array of nucleotides in a DNA molecule |
|
|
Term
|
Definition
| Stably integrating a piece of DNA into the genome of an organism |
|
|
Term
|
Definition
| Analyzing changes in an entire genome |
|
|
Term
|
Definition
| alters ONE codon so that it encodes a different amino acid |
|
|
Term
|
Definition
mutations that do not alter the amino acid sequence.
Mutations that occur in introns and non-genic regions are also silent |
|
|
Term
|
Definition
| mutation that alters one codon so that it now encodes for a STOP codon |
|
|
Term
| Nonsense suppressor mutation |
|
Definition
| mutation that allows the tRNA to recognize a nonsense codon and base pair with it.The gene continues to be transcribed. |
|
|
Term
|
Definition
| description of the number, type, and distances between Restriction sites on a piece of DNA |
|
|
Term
|
Definition
The length of the DNA can be accurately determined by allowing the charged DNA to run through an agarose gel.
DNA moves towards the Positive electrode.
The rate of migration of a DNA fragment is inversely proportional to its size.
Larger the size, slower its movement. |
|
|
Term
|
Definition
| Plasmids are naturally occurring circular pieces of DNA in E. coli |
|
|
