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Genetic lab exam
Midterm 1-5
35
Other
Not Applicable
02/19/2009

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Term
concept of chance
Definition
A force assumed to cause events that cannot be foreseen or controlled. ex: flipping coin
Term
addition principle
Definition
the probability of either one or the other of two mutually exclusive events occuring
Term
what is the purpose of chi square test
Definition
to determine whether experimentally obtained data constitute a good fit to a theoretical, expected ratio
Term
formulat for chi square
Definition
X2 = sum of (O-E)2 / E
Term
What is the critical point of critical value in chi square?
Definition
5%, or 3.841
Term
Who is responsible for development of D. melanogaster?
Definition
Thomas Hunt Morgan
Term
Who is Alfred A. Stutevant
Definition
showed the frequency of recombination between two linked genes and the distance
Term
Why use D. melanogaster?
Definition
short generation time, copious offspring, simple culturing requirements, well-defined genetics
Term
what is metabolism
Definition
sum of al physical and chemical changes that occur in an organism
Term
What is anabolism
Definition
process that use energy to produce materials from simple substrates.
Term
what is catabolism
Definition
process involve degradation of complex compounds with release of energy
Term
who sugested te relationship between genes and enzymes?
Definition
Archibald garrod
Term
Who said one gene one enzyme?
Definition
Beadle and tatum
Term
Who studied alkaptonuria
Definition
Archibald garrod
Term
what is alkaptonuria
Definition
a rare inherited metabolic discorder characterized by secretion of homogenistic acid in urine.
Term
what is ommatidia?
Definition
multiple visual structures of each eye in fruit fly
Term
what pteridine and ommochrome pigments make?
Definition
red and brown
Term
mutation in brown(bw)
Definition
disrupt biosynthesis of the pteridine --> brown eyes
Term
mutation in scarlet(st)
Definition
scarlet eyes
Term
vermilion(vn) and cinnabar(cn)
Definition
bright red eye
Term
stages of flies
Definition
egg--> instar --> larva --> pupil --> flies
Term
imago
Definition
slender, elongated, and light in color
Term
chromosome of flies
Definition
X, 2, 3, 4
Term
difference between females and males
Definition
females: elongated. males: round
Term
sternites
Definition
male. dark heavy bristials
Term
which of the following are sex linked
Definition
white eyes, scalloped wings, and yellow body
Term
what are the sizes for pipettes
Definition
P-20, P-200, P-1000 microliter
Term
intercalating agent
Definition
Ethidium Bromide. It is flat and stack between flat cyclic rings of bases
Term
UV thymine dimer
Definition
fuse adjacent tymine, and it is incapable of pairing polymerase. falls off during replication
Term
cation migrate to cathode(-)
Definition
anion migrates to anode(+)
Term
what's in the agarose Gel Electrophoresis
Definition
1% agarose, buffer, ethidium bromide
Term
How does DNA electrophoresis work?
Definition
electricual current moves the DNA and separate them on size, charge, and shape
Term
which one moves faster, supercoiled or linear fragment
Definition
supercoil
Term
what kind of dye?
Definition
bromophenol blue, migrates along with DNA fragments. Fluoresces strongly with exposed to UV light
Term
what are restriction enzymes
Definition
endonucleases which catalzye the cleavage of phosphate bonds within the starnds of DNA
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