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Fungi: Aseptic Techniques
Micro Lab
11
Microbiology
Undergraduate 3
03/01/2011

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Cards

Term
Characteristics of Fungi
Definition

  • Eukaryotic (higher) microorganisms 
  • Non-photosynthetic 
  • Lack Tissue differentiation
  • Cell walls made of chitin or other polysaccharides
  • Dimporhic- most have yeast-like and mold like form 

Term
Dimorphic
Definition

  • Having yeast and mold like form
  • Characteristic of Fungi 

 

Term
Basis of Classification of Fungi
Definition

  • The secxual spore structure
  • Mycelial septation
  • asexual spore type 

Term
4 TYPES OF FUNGI
Definition

  • Ascomyetes
  • Basidiomycetes
  • zygomycetes
  • Deuteromycetes 

Term

Deutermycetes

 

Definition

  • Only Class w/o known sexual spore stage
  • "Fungi Imperfecti" 

Term
Asexual Fungal Spores 
Definition

  • Sporangiospores: Rhizopus- sacs hold spores
  • Conidiospores: Penicillium, Aspergillus- spores made on specific hyphae 

Term
Asexual Yeast Production: 
Definition

  • Yeast exists as single cells 
  • Reproduce by budding off a mature cell 
Budding cells= blastospores 

Term
Aseptic Techniques
Definition

  • Sterilize media/ containers used for bac. growth
  • Keep containers covered
  • Sterilize materials used for handling cultures
  • Don't contaminate yourself
  • Properly discard of all material

Term
Sterilization: Physical Methods 
Definition

 

  1. Heat Methods
    1. Incineration
    2. Autoclaving media
  2. Irradiation methods
    1. UV light
  3. Filtration Methods
    1. HEPA filters 
    2. Membrane Filters

 

Term
Sterilization: Chemical Methods
Definition

  1. Disinfection/Sanitizing: Application to surfaces
  2. Gas Sterilization: Used for heat sensitive solid materials(plastics)
  3. Antibiotics: Narrow or broad ranges of microbial targets 

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