Term
| what is involves in activating and liscencing of DNA replication |
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Definition
ORC=find the origin
Cdc6 + Cdt1= load helicase MCM
CDK and DDK phosphorilate ORC protein and MCM |
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Term
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Definition
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Term
| what does Topo 1 do and what inhibits its |
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Definition
Topo 1= cut 1 strand, let it unwind and reseal that 1 strand.
campthothecin inhibits topo 1 |
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Term
| what does Topo 2 do and what inhibits its |
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Definition
Topo 2= cut 2 strand, let them unwind and reseal these strands.
Etoposide inhibits topo 2 |
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Term
| inhibitors of dna replication are most likely to ind medical uses as |
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Definition
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Term
| what does DNA polymerase a do |
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Definition
| builts a RNA primer at 5' if the new DNA stands |
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Term
| what do polymerase s and e need |
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Definition
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Term
| Whats the leading strand? what poly is associated with it |
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Definition
pol e= leading strand
leading strand = new synthesized DNA replicated towards the replication fork |
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Term
| Whats the lagging strand? what poly is associated with it |
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Definition
pol s= lagging strand
Lagging=synthesized away from the replication fork and is discontinous: OKAZAKI fragments |
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Term
| what does Proliferating cell nuclear antigen (PCNA) do |
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Definition
| keeps the DNA poly on the DNA |
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Term
| what kinds of bonds are made in new DNA strand |
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Definition
| covalent, H bonds and hydrophobic |
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Term
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Definition
| seals up the nicks or okazaki fragments by forming a phosphodiester bond |
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Term
| how can a RNA primer be removed from a DNA strand |
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Definition
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Term
| RNA telomeres cant replicate by the usual mechanism. what is used in order to integrate them |
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Definition
Telomerase integrates RNA template.
Its a RNA dependant DNA polymerase |
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Term
| gives the steps of replication |
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Definition
1- liscensing and activating: (ORC, phosphorilation)
2-replication fork ( DNA helicase, topo,SSBs)
3-DNA polymerase a binds to primer
4-DNA pol e binds to leading
DNA pol s binds to lagging |
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Term
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Definition
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Term
| what is a okazaki fragment |
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Definition
| short fragment of DNA (with no RNA primer at the 5' terminus) created on the lagging strand during DNA replication. |
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