Term
| First Step in Spills Procedure |
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Definition
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Term
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Definition
| Directs light rays onto and through the specimen |
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Term
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Definition
| Once the object is in focus with lowest power, it should be almost in focus with highest power |
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Term
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Definition
| Distance between the slide and bottom of lens |
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Term
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Definition
| Ability to see details, can be improved with oil |
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Term
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Definition
Sterilize loop
Select colony
Pick up and mix w/ saline
Heat Fix |
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Term
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Definition
Flood w/ crystal violet for 1 min
Flood with iodine for 1 min |
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Term
| Gram Stain: Decolorization |
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Definition
Drip acetone across smear till clear
Rinse |
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Term
| Gram Stain: Counter Stain |
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Definition
Flood w/ Safranin for 1 min
Rinse
Blot dry with bibulous paper |
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Term
| Most Critical Step in Gram Stain Procedure |
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Definition
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Term
| Color after prepared smear for gram stain |
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Definition
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Term
| Color after staining in gram stain |
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Definition
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Term
| Color after decolorization in gram stain |
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Definition
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Term
| Color after counterstain in gram stain |
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Definition
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Term
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Definition
Crystal violet left on to long
Both purple |
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Term
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Definition
Acetone left on to long
Both red |
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Term
| What part of cell determines gram stain reaction |
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Definition
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Term
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Definition
| Presence of more than one bacterial species |
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Term
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Definition
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Term
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Definition
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Term
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Definition
| Destroy or remove contaminants or microorganisms |
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Term
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Definition
| Destroys or removes all microorganisms, including endospores and viruses |
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Term
| Viruses most resistant to Decontamination |
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Definition
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Term
| What tests are used to identify Staphylococcus? |
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Definition
Mannitol Salt Agar
Coagulase Test
Staphaux Aggluation |
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Term
| Staphylococcus Sp. has what catalase reaction? |
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Definition
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Term
| Staphylococcus sp. has what gram stain reaction? |
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Definition
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Term
| Strepylococcus sp. has what catalase reaction? |
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Definition
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Term
| Strepylococcus has what gram stain reaction? |
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Definition
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Term
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Definition
minimum inhibitory concentration
lowest amount of antibiotic that inhibits growth of an organism |
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Term
| Direct Immunology testing |
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Definition
| measures presence of antigen |
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Term
| Indirect immunology testing |
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Definition
| Measures presence of antibody |
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Term
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Definition
ELISA
Western Blot-more specific |
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Term
| Blood Agar Plate has 5% of what? |
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Definition
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Term
| BAP is what kind of plate? |
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Definition
| Selective, differential and enriched |
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Term
| Color of Alpha, Beta, and Gamma Hemolysis |
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Definition
alpha-green
beta-clear
gamma-none |
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Term
| Mannitol Salt Agar turns yellow for what organism |
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Definition
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Term
| Mannitol Salt Salt agar has what indicator |
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Definition
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Term
| MSA grows what gram stains |
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Definition
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Term
| MacConkey grows what gram stain |
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Definition
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Term
| MacConkey has what indicator |
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Definition
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Term
| Lactose fermenters turn what color on MacConkey |
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Definition
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Term
| MacConkey grow what kind of organisms |
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Definition
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Term
| Hektoen grows what kind of organisms and what gram stain |
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Definition
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Term
| Hektoen has what indicator |
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Definition
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Term
| What organisms grow blue-green colonies on Hektoen? |
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Definition
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Term
| What kind of organisms will turn yellow on Hektoen? |
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Definition
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Term
| Bile Esculin will turn black from what organism? |
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Definition
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Term
| Bile esculin grows what gram stain? |
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Definition
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Term
| What disease is caused by Clostridium difficille? |
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Definition
| Pseudomembranous entercolitis |
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Term
| Neisseria gonorrhoeae has what form and gram stain |
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Definition
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Term
| Staphylococcus aureus has what catalase, coagulase reaction |
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Definition
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Term
| Yeast is what organism and gram stain |
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Definition
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Term
| Simmon's Citrate has what indicator |
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Definition
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Term
| What grows on chocolate media |
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Definition
Neisseria gonorrhea
5% to 10% CO2 |
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Term
| Oxidase positive is what color |
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Definition
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Term
| Psedemonas aurginosa can produce what color pus |
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Definition
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Term
| What is substrate of catalase test |
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Definition
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Term
| Streptococcus pneumoniae has what gram stain reaction and morphology |
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Definition
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Term
| What disk and what is used in that disk to test for Steptococcus pneumoniae and what type of hemolysis |
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Definition
P-Disk
Optochin
Alpha hemolytic |
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Term
| Steptococcus pyogenes has what type of hemolysis and what is its full name |
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Definition
Beta
Beta-hemolytic Strptococcus pyogenes group A |
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Term
| What disk and what is in that disk to test for strptococcus pyogenes |
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Definition
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