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Final Practical
Fluid Cell Count with Neubeur Hemocytometer
11
Science
Graduate
04/07/2013

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Cards

Term
what volume does the hemocytometer hold?
Definition
0.9mm^3
Term
calculation for total cells/mm^3
Definition
(cells counted x reciprocal of dilution factor)/ area x depth
Term
Pleural cavity procedure
Definition
thorecentesis
Term
pericardial cavity procedure
Definition
percaridial aspiration
Term
peritoneal cavity procedure
Definition
paracentesis
Term
joint space (synovial fluid) procedure
Definition
joint aspiration (artherocentasis)
Term
Spinal cord (CSF) procedure
Definition
spinal tap, lumbar puncture
Term
how can you tell if it is a traumatic tap?
Definition
high RBC count in first tube
Term
Perform visual exam on body fluid specimens.
Definition
Visual exam on body fluid specimens using clarity and color: Clarity- clear, cloudy, or bloody, color- CSF: colorless, slightly xanthrochromic, xanthrochromic, serous fluid: colorless, yellow, green, brown, milky white
Term
Perform hemocytometer cell count on body fluid specimens
Definition
Hemocytometer cell count- clean hemocytometer and cover slip, apply cover slip, and then apply a drop of fluid to each side of the grooves, let sit for a minute. Place the hemocytometer under 10X power and find the arrow, start at the top right corner of the grid. Count under 40X.
Term
Calculate red and white blood cells/┬ÁL based on variables such as squares counted (neubauer hemocytometer) and dilution factor of specimen.
Definition
Count/0.9 = cells/uL if using the entire grid, or cell/0.1 = cell/uL if using center grid only
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