Term
| Correctly perform a titer on a pre-natal specimen |
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Definition
| Label three tubes with patient’s name and dilution (1:2, 1:4, 1:8), and then add 100uL of saline to the bottom of each tube. Add 100uL of patient into the 1:2 tube. Mix contents and then transfer 100uL into the tube labeled 1:4, continue onto the next tube. Then add 1 drop of indicator cells to each tube. Mix and incubate for 60 minutes. Wash the tubes 4 times and add 2 drops of anti-IgG and centrifuge for 15 seconds. Read and Record results. To negative tubes add 1 drop of check cells. The last cell where there is agglutination is your end point. |
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Term
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Definition
| The last cell where there is agglutination is your end point. |
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Term
| When interpreting a titer, what criteria is used to determine when a woman is sensitized? |
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Definition
| A single fold increase or two or more consecutive two-fold increases in the antibody titer indicates sensitization. |
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Term
| When performing ABORh on a cord specimen, which reagents are needed? |
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Definition
| For an ABORh on a cord specimen you can only do a forward type and a Rh type. You need the anti-A, anti-B, anti-A,B antisera for the forward type, and the anti-D and the Gamma Clone control for the Rh type. |
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Term
| Why should cord cells be washed? |
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Definition
| Cord cells should be washed because of the presence of Wharton’s jelly which is a thick substance that can coat red blood cells and cause false agglutination |
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Term
| How many times should the cells be washed prior to testing? |
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Definition
| Cells should be washed 6-8 times. |
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