Term
| G eneral characteristics of fungi |
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Definition
| Chitin in cell wall, ergosterol in cell membrane, reproduction by means of spores, no chlorophyll, heterotrophic and not susceptible to antibacterial agents. |
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Term
| Explain the purpose of cyclohexiamide in fungal media |
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Definition
| Cyclohexiamide is an antibiotic for inhibiting protein synthesis of saprobic fungi on sabouraud dextrose agar |
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Term
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Definition
(botany) a slender tubular outgrowth from a spore in germination.
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Term
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Definition
| KOH dissolves human cellular elements and debris, which allows for easier visualization of fungal elements |
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Term
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Definition
| Calcoflour binds to chitin in fungal cell wall, fluorescence occurs at 400nm, fungal elements fluoresce blue-white |
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Term
| In vitro hair penetration test |
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Definition
| some fungi will penetrate the hair shaft, Trichophyton mentagrophytes is positive, Trichophyton rubrum is negative |
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Term
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Definition
| take mold from agar plate, and put it on a slide with a drop of lactophenol blue, used to identify morphologic structures of mold |
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Term
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Definition
| cut a small block of sterile agar, place on a sterile slide in a huminidity chamber, inoculate the four corners of the agar with fungi and apply a coverslip to the top of the agar. Ater incubation remove coverslip and palce on a new slide with a drop of lactopenal blue, observe for morphologic characteristics. |
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Term
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Definition
| asexual reproduction of yeast, budding yeast cells from weakened out-pouching of cell wall |
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Term
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Definition
| specialized hyphal structure that serves as a stalk on which conidia are formed |
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Term
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Definition
| mould characteristic, growing hyphae that become intertwined to form a loose network that can penetrate the substrate to obtain necessary nutrients |
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Term
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Definition
| yeast buds elongate, fail to dissociate, and form subsequent buds, looks like a link of sausage, cell wall constrictions rather than septations |
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Term
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Definition
| root-like structures that appear and anchor the organism to the agar surface, used in identification of zygomycetes |
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Term
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Definition
| living on dead or decayed organic mater, characteristic of most of the fungal organisms that cause human disease |
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Term
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Definition
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Term
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Definition
| supporting structure at which each sporangium is formed at the tip of, usually connected to one another by septate hyphae |
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Term
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Definition
| A minute, typically one-celled, reproductive unit capable of giving rise to a new individual without sexual fusion |
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Term
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Definition
| presence of pigmentation within the hyphae of molds |
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Term
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Definition
| absence of pigmentation within the hyphae of molds |
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Term
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Definition
| can grow as yeast at body temp and as mould at room temp |
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Term
| Clinical significance of dimorphism- |
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Definition
| allows organism to survive and infect humans inside their bodies as opposed to subcutaneous |
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Term
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Definition
| Histoplasma capsulatum, Blastomyces dermatitidis, Coccidioides immitis, paracoccidiodes brasiliensis, Sporothrix schenckii, and Penicillium marneffei |
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Term
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Definition
| fungal infection that causes disease |
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Term
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Definition
| toxic secondary disease caused by toxin from fungi |
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Term
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Definition
| usually on hands and feet, chronic infection characterized by swollen tumor-like lesions that yield granular pus through draining sinuses, the granules are masses of compact mycelia |
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Term
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Definition
| infections of the skin or nails caused by dermatophyte fungi, outer ring is active, progressing infection, with central healing within the ring |
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Term
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Definition
| subcutaneous mycoses in which there is a localized cutaneous and subcutaneous infection where the tissue turns into rounded sclerotic bodies, called copper pennies |
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Term
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Definition
| term to describe any infection caused by a dematiacious organism, can be subcutaneous, localized, or systemic infections |
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Term
| How do you tell a yeast from a mould? |
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Definition
| Yeast are single vegatative cells called blastoconidia, while moulds are filamentous fungi that can have mycelium, hyphae and rhizoids |
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Term
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Definition
| asexual reproduction that occurs on the side or end of hyphae or conidiophore |
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Term
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Definition
| larger of 2 types of conidia in a fungus that produces 2 sizes of conidia |
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Term
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Definition
| smaller of 2 types of conidia in a fungus that produces 2 sizes of conidia |
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Term
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Definition
| a cell that produces & extrudes conidia; tapers & lengthens with each condidum |
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Term
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Definition
| an asexual spore formed by the breaking of hyphae at the point of septation |
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Term
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Definition
| a cell that produces & extrudes conidia; does not taper or lengthen with each condidium |
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Term
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Definition
| asexual spore produces within a sporangium |
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Term
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Definition
| a closed sac-like structure in which asexual spores are formed by cleavage |
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Term
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Definition
| sexual reproduction occurs with the production of zygospores |
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Term
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Definition
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Term
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Definition
| sexual spores produced within a sac-like structure knowns as an ascus |
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Term
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Definition
| Microsporum, Trichophyton, Pseudallescheria |
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Term
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Definition
| sexual spore formed on a structure known as a basidium |
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Term
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Definition
| Filobasidiella neoformans (Cryptococcus neoformans) |
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Term
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Definition
| no known sexual mode of reproduction |
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Term
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Definition
| confined to the outermost layer of skin or hair |
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Term
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Definition
| affect the keratinized layer of skin, hair or nails |
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Term
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Definition
| involve deeper skin layers, including muscle & connective tissue |
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Term
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Definition
| affect internal organs or deep tissues of the body |
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Term
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Definition
Hair
Skin
Nails
Blood & bone marrow
CSF
Abscess fluids & wound exudates
Respiratory specimens
Urogenital & fecal specimens |
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Term
| Ways to perform direct microscopic examination (4) |
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Definition
KOH preparations
KOH with calcofluor white
India Ink
Tissue stains |
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Term
| Antibiotics used on media |
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Definition
Chloramphenicol (bacteria)
Cyclohexamide (saprobic fungi) |
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Term
| Incubation for fungal media |
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Definition
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