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FHDT
2 - Microscopy
7
Accounting
Kindergarten
01/09/2013

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Cards

Term
Maximum resolving power of light microscope?
Definition
-0.2 µm
-A RBC is about 6-8µm
Term
Name two fixatives and what they react with?
Definition
-Formaldehyde and glutaraldehyde
-react with amine groups
Term
Steps for embedding? Two types and advantages?
Definition
-Dehydration by bathing with a graded series of ethanol mixtures (from about 70-100%)
-Then ethanol is replaced by solvent miscible with embedding medium
-Then we embed with either paraffin or resin

-Resin can be run at a lower temp using a plastic solvent and so is subject to less shrinking
Term
Phase-contrast microscopy?
Definition
-Relies on fact that light changes "phase" when going through a medium; peaks change position
-allows study of live organisms by avoiding dyes

-interference microscopy uses a similar method
Term
Polarizing microscopy?
Definition
-uses polarized light to illuminate only structures with oriented molecules like cellulose, collagen, etc.
Term
Confocal microscopy?
Definition
-Thin plane of light is moved over a specimen and a computer reconstructs a surface map
Term
Fluorescence microscopy? What is the dye?
Definition
-Use acridine orange which complexes with DNA and RNA becoming yellow with DNA and reddish orange with RNA (stains cytoplasm)
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