Term
| Describe the macroconidia associated with Epidermophyton? |
|
Definition
|
|
Term
| What is the recommended temperature & the length of time for incubating fungal cultures? |
|
Definition
|
|
Term
| What is the preferred temperature for growing M. marinum, M. haemophilum & M. ulcerans |
|
Definition
|
|
Term
| What is the preferred temperature for growing M. tuberculosis? |
|
Definition
|
|
Term
| Which mycobacterial media are considered broth media? |
|
Definition
| 7H9, 7H12, 7H13, & Dubos Tween albumin |
|
|
Term
| What is the rate of growth that catorgizes an organism as a "rapid grower"? |
|
Definition
|
|
Term
| Identify organisms considered to be "rapid growers" |
|
Definition
| M. fortuitum-chelonae-abscessus |
|
|
Term
| Identify the reagents used in the Ziehl-Neelson stain. |
|
Definition
| Heated carbolifuchsin, acid alcohol, & methylene blue |
|
|
Term
| Identify the following organism: narrow hyphae w/ septations & dichotomous branching; closed fist appearance |
|
Definition
|
|
Term
| Which mold can be acquired by inhalation and form a "fungus ball" in old lung cavities? |
|
Definition
| Aspergillus species, primarily fumigatus |
|
|
Term
| Which fungi are endemic in Arkansas? |
|
Definition
| Blastomyces & Histoplasma |
|
|
Term
| Identify the following organism: broad based budding yeast (figure 8) on KOH mount |
|
Definition
|
|
Term
| Identify the following organism: germ tubes present |
|
Definition
|
|
Term
| Identify the following organism: blastocondidia & pseudohyphae present on cornmeal |
|
Definition
|
|
Term
| Identify the following organism: chlamydospores, blastocondidia & pseudohyphae present on cornmeal |
|
Definition
|
|
Term
| Identify the following organism: thick walled spherule w/ endospores on KOH & alternating arthroconidia on LPCB mount |
|
Definition
|
|
Term
| Which fungus is endemic in the American Southwest? |
|
Definition
|
|
Term
| Identify the following organism: capsule on India ink & black colonies on birdseed/niger seed agar (phenol oxidase present) |
|
Definition
|
|
Term
| Which yeast is an opportunistic organism isolated from AIDS patients? |
|
Definition
|
|
Term
| Which test has replaced the India ink mount on CSF? |
|
Definition
| Cryptococcus antigen test - more sensitive |
|
|
Term
| Identify the following organism: small, intracellular yeasts on Giemsa stain & tuberculate macroconidia/teardrop microconidia on LPCB mount |
|
Definition
|
|
Term
| Which fungus like to reside in bird & bat dung? |
|
Definition
|
|
Term
| Identify the dimorphoric fungi. |
|
Definition
| Histoplasma, Blastomyces, Coccidioides, Paracoccidiodes, & Sporothrix |
|
|
Term
| Identify the following organism: nonchromogen, slower grower, & most tests are = |
|
Definition
|
|
Term
| What is the most commonly isolated mycobacteria species in the US? |
|
Definition
|
|
Term
| Identify the following organism: slow grower, niacin & nitrate =, & T2H sensitive |
|
Definition
|
|
Term
| Identify the following organism: rapid grower, arylsulfatase +, & growth on special MAC |
|
Definition
|
|
Term
| What disease does M. fortuitum-chelonae cause? |
|
Definition
|
|
Term
| What disease does M. abscessus cause? |
|
Definition
|
|
Term
| Which organism is considered the tap water scotochromogen? |
|
Definition
|
|
Term
| Identify the following organism: scotochromogen, nitrate = & Tween 80 + |
|
Definition
|
|
Term
| Which organisms are considered nonchromogens? |
|
Definition
| M. haemophilum & M. avium complex |
|
|
Term
| Which organisms are considered photochromogens? |
|
Definition
|
|
Term
| Which mycobacteria species can not be grown in lab media? |
|
Definition
|
|
Term
| How can M. leprae be grown? |
|
Definition
|
|
Term
| Which organisms are considered scotochromogens? |
|
Definition
| M. scrofulaceum & M. gordonae |
|
|
Term
What is the ID for the following organism?
BACTEC bottle + NAP = GI 20
BACTEC bottle w/o NAP = GI 75 |
|
Definition
|
|
Term
| Identify the following organism: niacin & nitrate +, 68 C catalase =, & buff, rough colonies |
|
Definition
|
|
Term
| Identify the following organism: "spaghetti & meatballs" (yeast & hyphae) present on KOH mount |
|
Definition
|
|
Term
| Identify the following organism: "mariners wheel" yeast on KOH mount |
|
Definition
| Paracoccidioides brasiliensis |
|
|
Term
| Identify the following organism: narrow hyphae w/ septations; open fist appearance |
|
Definition
|
|
Term
| Identify the following organism: cigar-shaped yeast on KOH mount & "flowerette" arrangement on LPCB mount |
|
Definition
|
|
Term
| Name a nonpathogenic AFB species. |
|
Definition
|
|
Term
Identify the functions of the reagents used in the fluorescent stain:
Auramine-o-rhodamine (AOR)
Acid alcohol (AA)
Potassium permanganate (PP) |
|
Definition
| AOR-stains, AA-decolorizer, & PP-quences background fluorescence |
|
|
Term
| What is the principle of the VersaTrek (formerly ESP Myco) system? |
|
Definition
| Measures pressure changes (manometric system) |
|
|
Term
| How do the interferon-gamma release assays differ from the TB skin test? |
|
Definition
| Measures release of interferon gamma by blood WBCs to MTB antigens |
|
|
Term
| What is the rate of growth that catorgizes an organism as a "slow grower"? |
|
Definition
|
|
Term
|
Definition
| Above the surface - usually support reproductive structures |
|
|
Term
| Actinomycotic mycetoma are caused by ____________. |
|
Definition
| Actinomycetes, like Nocardia, Streptomyces, or Actinomadura |
|
|
Term
| Since Malassezia furfur requires fatty acids for growth, what is a practical means of culturing the organism? |
|
Definition
| Add olive oil to fungus medium |
|
|
Term
| What are the recommended collection procedures for isolating fungi from sputum? |
|
Definition
| AM sputum on 3 successive days |
|
|
Term
|
Definition
| Arthroconidia found inside hair shaft |
|
|
Term
|
Definition
| Arthroconidia found outside of hair shaft |
|
|
Term
| Identify the reagents used in the fluorescent stain. |
|
Definition
| Auramine-o-rhodamine, acid alcohol, & potassium permanganate |
|
|
Term
| What is the principle of the Septi-Chek AFB system? |
|
Definition
|
|
Term
| What is the disease caused by Piedraia hortae? |
|
Definition
|
|
Term
| What is the purpose of using KOH? |
|
Definition
| Breaks down cellular material; thus allows the viewing of fungal elements |
|
|
Term
| Describe the appearance of a positive 68 catalase. |
|
Definition
|
|
Term
| Describe the appearance of MTB on LJ medium. |
|
Definition
|
|
Term
| Identify the reagents used in the Kinyoun stain. |
|
Definition
| Carbolfuchsin, acid alcohol, & methylene blue |
|
|
Term
| How should normally sterile body fluids be processed for mycobacteria? |
|
Definition
| Centrifuged & sediment used for stains & media |
|
|
Term
Identify the functions of the reagents used in the Kinyoun & Ziehl-Neelson stains:
Carbolfuchsin (CF)
Acid alcohol (AA)
Methylene blue (MB) |
|
Definition
| CF (staining agent), AA (decolorizer), & MB (counterstain) |
|
|
Term
| Which disease causes "cauliflower-like lesions" usually found on extremities? |
|
Definition
|
|
Term
| What are the recommended collection procedures for isolating mycobacteria from urine? |
|
Definition
| Collect AM specimens; 3-5 over several days; 5-10 mls |
|
|
Term
| What are the recommended collection procedures for isolating mycobacteria from sputum? |
|
Definition
| Collect AM sputum samples; 3-5 over several days; 5-10 mls |
|
|
Term
| Which tests are used to identify the dimorphic molds? |
|
Definition
| Conversion of yeast to mold form, exoantigens, & NA probes (method of choice) |
|
|
Term
|
Definition
| Crosswalls between fungal cells |
|
|
Term
| What is the best way to diagnose cryptococcal meningitis? |
|
Definition
|
|
Term
| Describe how to perform an India ink mount. |
|
Definition
| CSF + India ink; coverslip & read |
|
|
Term
| Which fungi infect skin, hair, & nails? |
|
Definition
| Dermatophytes - Microsporum, Trichophyton & Epidermophyton |
|
|
Term
| What is the purpose of using the TB skin test and/or the interferon-gamma release assays? |
|
Definition
| Detect TB infection and disease |
|
|
Term
|
Definition
| Filaments that are constricted at point of attachment - seen in some yeast |
|
|
Term
| Define vegetative hyphae. |
|
Definition
| Food-absorbing portion of the hyphae; grows into agar |
|
|
Term
|
Definition
| Found in yeast; produced by budding |
|
|
Term
| Eumycotic mycetoma are caused by ________. |
|
Definition
|
|
Term
| What is the advantage of testing unknown AFB organisms again NAP in the BACTEC system? |
|
Definition
| Gives quick preliminary ID of MTB complex |
|
|
Term
| What is the preferred temperature for growing M. fortuitum-chelonae-abscessus? |
|
Definition
| Grows at 35-37 & at 25-33 |
|
|
Term
| Which mycobacterial media are considered selective egg-based media? |
|
Definition
| Gruft modification of LJ & mycobactosel - LJ |
|
|
Term
|
Definition
| Hyphae & conidia appear black microscopically |
|
|
Term
| What are the required culture conditions when using Middlebrook media? |
|
Definition
| Incubate in CO2, protect from light, & hold for only 4 weeks |
|
|
Term
| Describe how to perform a chlamydospore test. |
|
Definition
| Inoculate cornmeal agar; coverslip & incubate; examine for chlamydospores |
|
|
Term
| How should granules be processed for organisms? |
|
Definition
| Inoculate on appropriate media |
|
|
Term
| Describe the macroconidia associated with Microphyton? |
|
Definition
| Large, spindle-shaped, rough & thick-walled |
|
|
Term
|
Definition
| Large, usually multiseptate |
|
|
Term
| What disease does M. leprae cause? |
|
Definition
|
|
Term
| Define nonphotochromogen. |
|
Definition
| Light does not cause increase in pigment |
|
|
Term
|
Definition
| Lightly pigmented mold (shades of blue & green) observed microscopically |
|
|
Term
|
Definition
| Long strands of cells (tubular filaments) that develop from spores |
|
|
Term
| Describe how to perform lactophenal aniline (cotton) blue (LPCB) mount. |
|
Definition
| LPCB added to slide; add organism & coverslip; examine microscopically |
|
|
Term
| What disease does M. scrofulaceum cause? |
|
Definition
|
|
Term
|
Definition
| Many hyphae intertwined to form thick mat |
|
|
Term
| What is the principle of the BACTEC 460 system? |
|
Definition
|
|
Term
| What is the principle of the MB/BacT system? |
|
Definition
| Measures CO2 production w/ CO2 sensor & colorimetric sensor |
|
|
Term
| What is the principle of the BACTEC 9000 system? |
|
Definition
| Measures O2 changes using a fluorescent sensor |
|
|
Term
| What type of special media is required to culture M. haemophilum? |
|
Definition
| Media w/ hemin, hemoglobin, or ferric ammonium citrate - CHOC, BAP, etc |
|
|
Term
| What media is recommended for isolation of Cryptococcus neoformans? |
|
Definition
| Media without cycloheximide |
|
|
Term
| Which mycobacterial media are considered nonselective agar-based media? |
|
Definition
| Middlebrook agar: 7H10 & & 7H11 |
|
|
Term
| What is the recommended procedure for processing tissue for fungi? |
|
Definition
| Mince before inoculating media |
|
|
Term
| Which mycobacterial media are considered selective agar-based media? |
|
Definition
| Mitchison's selective 7H11 & mycobactosel-middlebrook 7H10 |
|
|
Term
| Which clues help determine whether an isolate is clinically significant or a contaminant? |
|
Definition
| MO observed microscopically in specimen or repeatedly isolated |
|
|
Term
| What are the recommended collection procedures for isolating fungi from sputum? |
|
Definition
| AM sputum on 3 successive days |
|
|
Term
|
Definition
| Arthroconidia found inside hair shaft |
|
|
Term
|
Definition
| Arthroconidia found outside of hair shaft |
|
|
Term
| Identify the reagents used in the fluorescent stain. |
|
Definition
| Auramine-o-rhodamine, acid alcohol, & potassium permanganate |
|
|
Term
| What is the principle of the Septi-Chek AFB system? |
|
Definition
|
|
Term
| What is the disease caused by Piedraia hortae? |
|
Definition
|
|
Term
| What is the purpose of using KOH? |
|
Definition
| Breaks down cellular material; thus allows the viewing of fungal elements |
|
|
Term
| Describe the appearance of a positive 68 catalase. |
|
Definition
|
|
Term
| Describe the appearance of MTB on LJ medium. |
|
Definition
|
|
Term
| Identify the reagents used in the Kinyoun stain. |
|
Definition
| Carbolfuchsin, acid alcohol, & methylene blue |
|
|
Term
| How should normally sterile body fluids be processed for mycobacteria? |
|
Definition
| Centrifuged & sediment used for stains & media |
|
|
Term
Identify the functions of the reagents used in the Kinyoun & Ziehl-Neelson stains:
Carbolfuchsin (CF)
Acid alcohol (AA)
Methylene blue (MB) |
|
Definition
| CF (staining agent), AA (decolorizer), & MB (counterstain) |
|
|
Term
| Which disease causes "cauliflower-like lesions" usually found on extremities? |
|
Definition
|
|
Term
| What are the recommended collection procedures for isolating mycobacteria from urine? |
|
Definition
| Collect AM specimens; 3-5 over several days; 5-10 mls |
|
|
Term
| What are the recommended collection procedures for isolating mycobacteria from sputum? |
|
Definition
| Collect AM sputum samples; 3-5 over several days; 5-10 mls |
|
|
Term
| Which tests are used to identify the dimorphic molds? |
|
Definition
| Conversion of yeast to mold form, exoantigens, & NA probes (method of choice) |
|
|
Term
|
Definition
| Crosswalls between fungal cells |
|
|
Term
| What is the best way to diagnose cryptococcal meningitis? |
|
Definition
|
|
Term
| Describe how to perform an India ink mount. |
|
Definition
| CSF + India ink; coverslip & read |
|
|
Term
| Which fungi infect skin, hair, & nails? |
|
Definition
| Dermatophytes - Microsporum, Trichophyton & Epidermophyton |
|
|
Term
| What is the purpose of using the TB skin test and/or the interferon-gamma release assays? |
|
Definition
| Detect TB infection and disease |
|
|
Term
|
Definition
| Filaments that are constricted at point of attachment - seen in some yeast |
|
|
Term
| Define vegetative hyphae. |
|
Definition
| Food-absorbing portion of the hyphae; grows into agar |
|
|
Term
|
Definition
| Found in yeast; produced by budding |
|
|
Term
| Eumycotic mycetoma are caused by ________. |
|
Definition
|
|
Term
| What is the advantage of testing unknown AFB organisms again NAP in the BACTEC system? |
|
Definition
| Gives quick preliminary ID of MTB complex |
|
|
Term
| What is the preferred temperature for growing M. fortuitum-chelonae-abscessus? |
|
Definition
| Grows at 35-37 & at 25-33 |
|
|
Term
| Which mycobacterial media are considered selective egg-based media? |
|
Definition
| Gruft modification of LJ & mycobactosel - LJ |
|
|
Term
|
Definition
| Hyphae & conidia appear black microscopically |
|
|
Term
| What are the required culture conditions when using Middlebrook media? |
|
Definition
| Incubate in CO2, protect from light, & hold for only 4 weeks |
|
|
Term
| Describe how to perform a chlamydospore test. |
|
Definition
| Inoculate cornmeal agar; coverslip & incubate; examine for chlamydospores |
|
|
Term
| How should granules be processed for organisms? |
|
Definition
| Inoculate on appropriate media |
|
|
Term
| Describe the macroconidia associated with Microphyton? |
|
Definition
| Large, spindle-shaped, rough & thick-walled |
|
|
Term
|
Definition
| Large, usually multiseptate |
|
|
Term
| What disease does M. leprae cause? |
|
Definition
|
|
Term
| Define nonphotochromogen. |
|
Definition
| Light does not cause increase in pigment |
|
|
Term
|
Definition
| Lightly pigmented mold (shades of blue & green) observed microscopically |
|
|
Term
|
Definition
| Long strands of cells (tubular filaments) that develop from spores |
|
|
Term
| Describe how to perform lactophenal aniline (cotton) blue (LPCB) mount. |
|
Definition
| LPCB added to slide; add organism & coverslip; examine microscopically |
|
|
Term
| What disease does M. scrofulaceum cause? |
|
Definition
|
|
Term
|
Definition
| Many hyphae intertwined to form thick mat |
|
|
Term
| What is the principle of the BACTEC 460 system? |
|
Definition
|
|
Term
| What is the principle of the MB/BacT system? |
|
Definition
| Measures CO2 production w/ CO2 sensor & colorimetric sensor |
|
|
Term
| What is the principle of the BACTEC 9000 system? |
|
Definition
| Measures O2 changes using a fluorescent sensor |
|
|
Term
| What type of special media is required to culture M. haemophilum? |
|
Definition
| Media w/ hemin, hemoglobin, or ferric ammonium citrate - CHOC, BAP, etc |
|
|
Term
| What media is recommended for isolation of Cryptococcus neoformans? |
|
Definition
| Media without cycloheximide |
|
|
Term
| Which mycobacterial media are considered nonselective agar-based media? |
|
Definition
| Middlebrook agar: 7H10 & & 7H11 |
|
|
Term
| What is the recommended procedure for processing tissue for fungi? |
|
Definition
| Mince before inoculating media |
|
|
Term
| Which mycobacterial media are considered selective agar-based media? |
|
Definition
| Mitchison's selective 7H11 & mycobactosel-middlebrook 7H10 |
|
|
Term
| Which clues help determine whether an isolate is clinically significant or a contaminant? |
|
Definition
| MO observed microscopically in specimen or repeatedly isolated |
|
|
Term
What is the ID for the following organism?
BACTEC bottle + NAP = GI 85
BACTEC bottle w/o NAP = GI 80 |
|
Definition
| Mycobacteria other than tuberculosis (MOTT) |
|
|
Term
| Why is the use of three or more drugs recommended to treat mycobacterial infections? |
|
Definition
| Mycobacteria readily become resistant when treated with single drug |
|
|
Term
| What reagents are used in the most common decontamination procedure? |
|
Definition
| N-acetyl-l-cystaine (NALC) & 2% NaOH |
|
|
Term
|
Definition
| No crosswalls between cells |
|
|
Term
| What diseases are caused Nocardia? |
|
Definition
| Nocardiosis (pulmonary) & mycetoma (granules present) |
|
|
Term
|
Definition
| Nonmotile, asexual reproductive structures - also known as spores |
|
|
Term
| Describe how to perform a germ tube test. |
|
Definition
| Organism + plasma; incubate; examine for tube-like structure |
|
|
Term
| What is the usual appearance of Nocardia on the modified acid-fast stain? |
|
Definition
|
|
Term
| While examining a Gram stain smear from a brain abscess, the MT sees Gram-positive branching rods. Before contacting the doctor, the technologist should ______________. |
|
Definition
| Perform a modified AF stain |
|
|
Term
| Which mycobacterial media are considered nonselective egg-based media? |
|
Definition
| Petragnani, American thoracic society (ATS), & Lowenstein-Jensen (LJ) |
|
|
Term
|
Definition
| Pigment in dark (= amount of pigment whether light-exposed or left in dark) |
|
|
Term
|
Definition
| Pigment production upon exposure to light |
|
|
Term
| What is the disease caused by Pneumocystis jiroveci? |
|
Definition
|
|
Term
| What disease does M. kansasii cause? |
|
Definition
|
|
Term
What is the ID for the following organism?
BACTEC bottle + NAP = GI 85
BACTEC bottle w/o NAP = GI 80 |
|
Definition
| Mycobacteria other than tuberculosis (MOTT) |
|
|
Term
| Why is the use of three or more drugs recommended to treat mycobacterial infections? |
|
Definition
| Mycobacteria readily become resistant when treated with single drug |
|
|
Term
| What reagents are used in the most common decontamination procedure? |
|
Definition
| N-acetyl-l-cystaine (NALC) & 2% NaOH |
|
|
Term
|
Definition
| No crosswalls between cells |
|
|
Term
| What diseases are caused Nocardia? |
|
Definition
| Nocardiosis (pulmonary) & mycetoma (granules present) |
|
|
Term
|
Definition
| Nonmotile, asexual reproductive structures - also known as spores |
|
|
Term
| Describe how to perform a germ tube test. |
|
Definition
| Organism + plasma; incubate; examine for tube-like structure |
|
|
Term
| What is the usual appearance of Nocardia on the modified acid-fast stain? |
|
Definition
|
|
Term
| While examining a Gram stain smear from a brain abscess, the MT sees Gram-positive branching rods. Before contacting the doctor, the technologist should ______________. |
|
Definition
| Perform a modified AF stain |
|
|
Term
| Which mycobacterial media are considered nonselective egg-based media? |
|
Definition
| Petragnani, American thoracic society (ATS), & Lowenstein-Jensen (LJ) |
|
|
Term
|
Definition
| Pigment in dark (= amount of pigment whether light-exposed or left in dark) |
|
|
Term
|
Definition
| Pigment production upon exposure to light |
|
|
Term
| What is the disease caused by Pneumocystis jiroveci? |
|
Definition
|
|
Term
| What disease does M. kansasii cause? |
|
Definition
|
|
Term
| What disease does M. avium complex cause? |
|
Definition
| Pulmonary - disseminates in immunocompromised patients |
|
|
Term
| Describe the appearance of a positive Tween 80 test. |
|
Definition
|
|
Term
| Describe the appearance of a positive arylsulfatase. |
|
Definition
|
|
Term
| Describe a positive Kinyoun/Ziehl Neelson stain. |
|
Definition
| Red rods against a blue background |
|
|
Term
| Positive & negative stain controls are run along with the patient slides. What is the appropriate procedure if the controls slides are not correct? |
|
Definition
| Restain & read the QC slides & patients slides before reporting any results |
|
|
Term
|
Definition
| Round, thick-walled resistant spores |
|
|
Term
|
Definition
|
|
Term
| Which body site is normally affected by M. haemophilum infections? |
|
Definition
| Skin & can disseminate in AIDS patients |
|
|
Term
|
Definition
| Small, unicellular spores |
|
|
Term
| Describe the macroconidia associated with Trichophyton? |
|
Definition
|
|
Term
| The natural reservoir for Blastomyces dermatitidis is thought to be __________. |
|
Definition
|
|
Term
| Describe how to perform a KOH mount. |
|
Definition
| Specimen + 10% KOH; coverslip & heat gently; read after 10-15 minutes |
|
|
Term
| Describe how to perform the calcofluor white stain. |
|
Definition
| Specimen + KOH + calcofluor white; examine w/ fluorescent microscope |
|
|
Term
| Which specimens should be decontaminated for mycobacteria isolation? |
|
Definition
| Specimens with normal flora |
|
|
Term
|
Definition
| Spores formed directly from the hyphae by framentation at septation points |
|
|
Term
| Which disease is known as the Rose gardener's disease? |
|
Definition
|
|
Term
|
Definition
| Stalk on which conidia develop |
|
|
Term
| When performing mycobacterial susceptibility testing, the number of colonies on test quadrants is compared with control quadrant. If the test growth is < 1% of the control growth, the organisms is _________; if greater or =, _________. |
|
Definition
| Susceptible (> 99% inhibition); resistant (< 99% inhibition) |
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|
Term
| What disease does M. marinum cause? |
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Definition
|
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Term
| What disease does M. bovis cause? |
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Definition
|
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Term
| What disease does M. tuberculosis cause? |
|
Definition
| TB (more likely in HIV & elderly) |
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Term
| Why are stains (such as the Giemsa stain) used to detect Histoplasma rather than the KOH mount? |
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Definition
| The organism is too small to see using a KOH mount. |
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Term
| What is the advantage of using the scotch tape method rather than the tease mount for identifying fungi? |
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Definition
| The spore arrangements are better preserved when using the scotch tape method |
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Term
| The names of diseases caused by dermatophyes are usually referred to as _____ (meaning ringworm). |
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Definition
|
|
Term
| What is the disease caused by Hortaea werneckii? |
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Definition
|
|
Term
| What is the disease caused by Malassezia furfur? |
|
Definition
| Tinea versicolor or pityriasis versicolor - causes superficial skin lesions |
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|
Term
| What is the purpose of using the Kinyoun/Ziehl-Neelson stain? |
|
Definition
| Typically used to confirm a positive fluorochrome stain |
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|
Term
| What disease does M. ulcerans cause? |
|
Definition
|
|
Term
| What is the recommended collection procedure for isolating fungi from urine? |
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Definition
|
|
Term
| What is the best way to diagnose Pneumocystis jiroveci? |
|
Definition
| Use of stains, such as Giemsa & GMS |
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|
Term
| What is the purpose of using the fluorescent stain? |
|
Definition
| Used by most labs as screening stain |
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Term
| How are subcutaneous mycoses acquired? |
|
Definition
| Usually inoculated into skin thru trauma splinter/thorn |
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|
Term
| How are dimorphic fungi acquired? |
|
Definition
| Usually thru inhalation of spores |
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|
Term
| Identify the following organism: structures resembling "copper pennies" on KOH mount |
|
Definition
| Variety of fungi that cause chromoblastomycosis |
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|
Term
| What is the disease caused by Trichosporon beigelii? |
|
Definition
|
|
Term
| What is the definition of dimorphic fungi? |
|
Definition
| Yeast at 37 (or in tissue) & mold at RT |
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|
Term
| What is the color of a positive niacin test? |
|
Definition
|
|
Term
| Describe a positive fluorescent stain. |
|
Definition
| Yellow to orange fluorescence against a black background |
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|
Term
| Identify the following organism: nonseptate hyphae w/ broad, irregular, ribbon-like appearance |
|
Definition
| Zygomycetes (Rhizopus, Mucor & Absidia) |
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