Term
Is the following considered an anaerobic GNB, GNC, GPC, or GPB:
Veillonella |
|
Definition
|
|
Term
Is the following considered an anaerobic GNB, GNC, GPC, or GPB:
Peptostreptococcus |
|
Definition
|
|
Term
Is the following considered an anaerobic GNB, GNC, GPC, or GPB:
Bacteroides |
|
Definition
|
|
Term
Is the following considered an anaerobic GNB, GNC, GPC, or GPB:
Prevotella |
|
Definition
|
|
Term
Is the following considered an anaerobic GNB, GNC, GPC, or GPB:
Porphyromonas |
|
Definition
|
|
Term
Is the following considered an anaerobic GNB, GNC, GPC, or GPB:
Fusobacterium |
|
Definition
|
|
Term
Is the following considered an anaerobic GNB, GNC, GPC, or GPB:
Clostridium (Spore former) |
|
Definition
|
|
Term
Is the following considered an anaerobic GNB, GNC, GPC, or GPB:
Eubacterium |
|
Definition
|
|
Term
Is the following considered an anaerobic GNB, GNC, GPC, or GPB:
Actinomyces |
|
Definition
|
|
Term
Is the following considered an anaerobic GNB, GNC, GPC, or GPB:
Bifidobacerium |
|
Definition
|
|
Term
Is the following considered an anaerobic GNB, GNC, GPC, or GPB:
Propionibacterium |
|
Definition
|
|
Term
| Which MO is considered as an Anaerobic diphtheroids |
|
Definition
|
|
Term
| With the Intestinal Tract what is the Anaerobes : Eenterics? |
|
Definition
|
|
Term
What type of Anaerobic specimen are the following considered:
Throat/Nasopharyngeal swab/Sputum
Feces/Bowel contents
Voided urine
Vaginal/Cervical Swab |
|
Definition
|
|
Term
| What are considered good specimens for Anaerobic collection? |
|
Definition
|
|
Term
| What is considered the lest desirable type of specimen for Anaerobic collection? |
|
Definition
|
|
Term
True or False:
Swab with Amies gel is approved for aerobic and anaerobic use |
|
Definition
|
|
Term
| What are Anaerobe BA, PEAK-K, Thio Broth, and Chopped Meat Broth supplemented with? |
|
Definition
|
|
Term
|
Definition
|
|
Term
| What is LKV or KVLB helpful in culturing? |
|
Definition
|
|
Term
| BBE - Isolation/Presumptive ID: ________ _____ group |
|
Definition
|
|
Term
True or False:
Thioglycollate is a reducing substance that keeps the environment anaerobic |
|
Definition
|
|
Term
True or False:
Thio needs to be boiled or steamed before use |
|
Definition
|
|
Term
| The Palladium catalyst is inactivated by what? |
|
Definition
|
|
Term
| The Palladium catalyst is reactivated by what? |
|
Definition
| Heating @ 160 degrees Celsius for 2 hours |
|
|
Term
| Where is the Palladium catalyst stored? |
|
Definition
|
|
Term
| Must incubate plates in GasPak for how many hours before opening? |
|
Definition
|
|
Term
| What is the Anaerobe gas composition? |
|
Definition
|
|
Term
| What type of loop should you use for general identification procedures? |
|
Definition
| Platinum or stainless steel loop |
|
|
Term
True or False:
Most GN Anaerobes are Resistant to Vancomycin |
|
Definition
|
|
Term
True or False:
Most GP MOs are Resistant to Colistin and Sensitive to Vancomycin |
|
Definition
|
|
Term
| What 3 things can you determine from Egg Yolk Agar? |
|
Definition
Lecithinase
Lipase
Proteolysis |
|
|
Term
| Positive Result for Lecithinase |
|
Definition
| Opaque zone formed when lecithin is destroyed |
|
|
Term
| Positive Result for Lipase |
|
Definition
Iridescent sheen
pearly layer
"oil on water" |
|
|
Term
| Positive Result for Proteolysis |
|
Definition
| Clear zone surrounding growth |
|
|
Term
| What is the Gram Stain of Bacteroides? |
|
Definition
|
|
Term
| Which MO is the most common cause of anaerobic infections? |
|
Definition
|
|
Term
Identify the following:
Kana - R
Vanc - R
Col - R
Bile - R
Black/Brown Pigment - = |
|
Definition
|
|
Term
Identify the following:
Kana - R
Vanc - R
Col - V
Bile - S
Black/Brown Pigment - + |
|
Definition
|
|
Term
Identify the following:
Kana - R
Vanc - S
Col - R
Bile - S
Black/Brown Pigment - + |
|
Definition
|
|
Term
Identify the following:
Kana - S
Vanc - R
Col - S
Bile - S
Black/Brown Pigment - = |
|
Definition
|
|
Term
| What is the gram stain typical of Prevotella? |
|
Definition
|
|
Term
| Is Prevotella a pigmenting species? |
|
Definition
| Yes, but there are several nonpigmented species |
|
|
Term
| Does Prevotella fluoresce red under ultraviolet light? |
|
Definition
|
|
Term
| What is the gram stain of Porphyromonas? |
|
Definition
|
|
Term
| Does Porphyromonas fluoresce red under ultraviolet light? |
|
Definition
|
|
Term
| What is the Gram Stain of Fusobacterium? |
|
Definition
| GNB, spindle shaped, long slender filaments |
|
|
Term
| What do the colonies of Fusobacterium look like? |
|
Definition
| Ground glass, speckled, or "bread crumb" |
|
|
Term
| What is Peptostreptococcus anaerobius sensitive to? |
|
Definition
| Sodium polyanethol sulfonate (SPS) |
|
|
Term
| Does Veillonella Fluoresce red under ultraviolet light? |
|
Definition
|
|
Term
| Propionibacterium acnes is most often associated with what? |
|
Definition
| Blood culture contamination |
|
|
Term
| What is the typical Indole reaction for Propionibacterium acnes? |
|
Definition
|
|
Term
| What is the typical Catalase reaction for Propionibacterium acnes? |
|
Definition
|
|
Term
| What does Actinomyces cause? |
|
Definition
|
|
Term
| Is Actinomyces considered normal flora? |
|
Definition
|
|
Term
| What is Actinomyces often associated with? |
|
Definition
|
|
Term
| Which MO may form "Sulfur Granules"? |
|
Definition
|
|
Term
| Which MOs gram stain morphology may branch (GPB - nonsporeformers) |
|
Definition
|
|
Term
| What is the colony characteristic of Actinomyces? |
|
Definition
|
|
Term
| Is Actinomyces a spore former? |
|
Definition
|
|
Term
| Is Propionibacterium acnes a spore former? |
|
Definition
|
|
Term
| Is Clostridium a spore former? |
|
Definition
|
|
Term
| What is the Gram Stain of Clostridium? |
|
Definition
GPB, Spore formers
Box Car Shape |
|
|
Term
| Is Clostridium perfringens considered normal flora? |
|
Definition
|
|
Term
| What 2 types of infections are often associated with Clostridium perfringens? |
|
Definition
|
|
Term
| Which MO is associated with a double zone of Beta hemolysis? |
|
Definition
|
|
Term
| Is Clostridium perfringens Reverse CAMP Positive or Negative? |
|
Definition
|
|
Term
| Is Clostridium perfringens Lecithinase Positive or Negative? |
|
Definition
|
|
Term
| Which MO is associated with "Antibiotic Associated Diarrhea?" |
|
Definition
|
|
Term
| Which MO is associated with Pseduomembranous colitis? |
|
Definition
|
|
Term
True or False:
Clostridium difficile is normal GI tract flora |
|
Definition
|
|
Term
| Only people with what should be tested for Clostridium difficile? |
|
Definition
|
|
Term
| What does Clostridium difficile produce? |
|
Definition
|
|
Term
| What type of special media do you use for Clostridium difficile? |
|
Definition
| Cycloserine-cefoxitin-egg yolk, fructose (CCFA) |
|
|
Term
| What color colonies does Clostridium difficile produce on CCFA? |
|
Definition
|
|
Term
| Isolates from CCFA must be tested for what? |
|
Definition
|
|
Term
| What tests can you run to detect toxins (A&B) &/or glutamate dehydrogenase (GDH)? |
|
Definition
|
|
Term
| Toxins A & B and glutamate dehydrogenase (GDH) are enzymes that are frequently associated with what MO? |
|
Definition
|
|
Term
| Stool (with toxin) + Tissue Cells ---> ? |
|
Definition
|
|
Term
| Stool (with toxin) + Antitoxin + Tissue Cells ---> ? |
|
Definition
|
|
Term
| Stool (No toxin) + Tissue Cells ---> ? |
|
Definition
|
|
Term
| What was once considered the gold standard for identification of Clostridium difficle? |
|
Definition
|
|
Term
| Which MO is tied with Bacteremia associated with underlying malignancy? |
|
Definition
|
|
Term
| Which MO is associated with causing Tetanus (toxin) and is difficult to culture? |
|
Definition
|
|
Term
| Which MO is associated with causing Botulism and whose diagnosis is usually by detecting toxin in patient specimen? |
|
Definition
|
|
Term
True or False:
Botulism is a type of neuroparalytic disease |
|
Definition
|
|
