Term
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Definition
| often a plasmid or viral DNA carries DNA segment that is to be cloned |
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Term
| Gene Cloning Process(Step 1 of Step 1)- Isolating vector DNA from bacterial cell does what? |
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Definition
| Isolates gene of interest from chromosomal DNA |
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Term
| Gene Cloning Process (Step 2 of 1) - Inserting gene of interest into vector does what? |
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Definition
| Produces "Recombinant Vector" |
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Term
| Gene Cloning Process (Step 3 of 1) - Introducing Recombinant Vector into host cell that doesn't already have a vector does what? |
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Definition
| Host cell copies vector and divides to produce many cells |
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Term
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Definition
| cut DNA at specific sequences |
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Term
| Gene Cloning Process - 3 Mini Steps of Step 2 |
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Definition
| 1. Cut vector and chromosomal DNA with restriction enzymes EcoRI. 2. Sticky ends hydrogen-bond w/ each other due to complimentary sequences. 3. DNA ligase is added. |
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Term
| Gene Cloning Process (Step 3 of 2) - What happens when DNA ligase is added? |
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Definition
| Closes gap (catalyzes formation of covalent bonds in DNA backbone) |
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Term
| Gene Cloning Process (Step 2 of 2) - What happens when sticky ends hydrogen-bond w/ each other due to complimentary sequences? |
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Definition
| Fragment of DNA carrying the gene of interest hydrogen bonds to vector |
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Term
| What happens when fragment of DNA carrying the gene of interest hydrogen bonds to the vector? |
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Definition
| 4 gaps where covalent bonds in DNA backbone are missing |
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Term
| What is the purpose of gene cloning? |
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Definition
| To survey all the genes in the genome of an organism or all the genes expressed in a certain tissue of an organism |
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Term
| How is the purpose of gene cloning achieved? |
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Definition
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Term
| What is the role of restriction endonucleases? |
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Definition
| They cut the DNA at specific sequences known as Restriction Sites |
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Term
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Definition
| Polymerase Chain Reaction |
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Term
| What uses heat resistant DNA polymerase (Taq polmerase)? |
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Definition
| Polymerase Chain Reaction |
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Term
| What does the PCR process amplify? |
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Definition
| DNA w/o bacterial cloning |
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Term
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Definition
| DNA is heated to separate strands |
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Term
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Definition
| Temp is lowered, which allows primers to bind to the Template DNA |
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Term
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Definition
| Incubation allows synthesis of a complimentary strand |
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Term
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Definition
| A gene is introduced into a plant or animal cell |
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Term
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Definition
| used to cut DNA at specific sequences |
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Term
| Sticky Ends on DNA Fragments |
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Definition
| Can be produced by restriction enzymes, joined together by DNA ligase, and have complementary sequences that can be hydrogen bonded together |
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Term
| When proteins are separated using gel electrophoreses, they move from the... |
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Definition
| Negative electrode to the positive electrode |
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Term
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Definition
| A protein, connects the cytoskeleton to the extracellular matrix (ECM) |
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Term
| After waiting 2 weeks to 6 months for a case study where dystrophin and a viral integrase were injected into mouse muscle, what happened? |
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Definition
| Negative integrase stayed the same. Positive integrase increased. |
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Term
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Definition
| Network of material secreted from cells (forms a complex of meshwork outside the cells) |
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