Term
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Definition
| Which of the following is not present in the core RNA polymerase? |
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Term
| B. Use the core enzyme of RNA polymerase. |
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Definition
| An experiment was designed to obtain nonspecific transcription from both strands of a DNA molecule. Which of the following strategies would be most effective in achieving this? |
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Term
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Definition
| Which of the following techniques would be most useful in testing the ability of the core RNA polymerase to bind to the promoter? |
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Term
| A. It does not have a DNA-binding domain. |
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Definition
| Which of the following statement is false regarding the sigma-factor? |
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Term
| A. The DNA is bound mainly to the sigma-subunit. |
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Definition
| Which of the following is true about the holoenzyme of RNA pol in an open complex? |
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Term
| D. add methyl groups to DNA. |
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Definition
| Dimethyl sulfate is used to |
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Term
| D. Polymerase complex would be loosely associated with the promoter. |
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Definition
| Which of the following would most likely occur if an in vitro transcription assay is preformed by using a cell extract containing an αsubunit without the C-terminal domain? |
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Term
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Definition
| Place the following in the order that they occur during transcription initiation: 1 - formation of open complex; 2 - formation of closed complex; 3 promoter clearance; 4 - synthesis of about 10 nucleotides |
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Term
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Definition
| Which of the following techniques is suitable for the study of DNA:RNA hybrid formation during transcription? |
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Term
| E. They contain an inverted repeat, they contain a hairpin loop, and they contain several T's in the nontemplate strand of DNA. |
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Definition
| Which of the following are characteristic of intrinsic terminator elements? |
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Term
| E. Rho has no helicase activity. |
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Definition
| Which of the following is incorrect regarding rho-dependent terminators? |
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Term
| E. Rho has no helicase activity. |
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Definition
| Which of the following is incorrect regarding rho-dependent terminators? |
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Term
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Definition
| Which of the following does not have a DNA-binding domain? |
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Term
| E. All of the choices are correct. |
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Definition
| Which of the following bond in lactose links between glucose and galactose? |
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Term
| B. b-galactosidase will be constitutively produced. |
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Definition
| Which of the following statements is true about a lac operon with the following genotype? I- Oc Z+ Y- and I- O+ Z- Y- |
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Term
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Definition
| Which of the following plasmids could be used to restore inducible regulation of b-galatosidase in this mutant: I+ Oc Z- Y+ A+? |
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Term
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Definition
| Which of the following organisms would produce functional lac products in the presence of IPTG? |
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Term
| B. The operon is nonrepressible. |
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Definition
| Which of the following statements is true about a lac operon with this genotype? I-d O+ Z+ Y- A- |
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Term
| C. run-off transcription assay |
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Definition
| Which of the following techniques is most useful in determining if RNA polymerase has initiated transcription from the lac DNA template? |
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Term
| C. The level of fluorescence would be very high in the assay buffer. |
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Definition
| In an in vitro transcription assay to detect the level of transcription from a lac operon, one of the RNA precursors was tagged with a fluorescent label on the γ-phosphate. Which of the following is a likely outcome if transcription occurred? |
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Term
| B. CAP will assist in stimulating transcription of the lac operon if lactose is added. |
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Definition
| Which of the following is mostly likely to occur if the level of glucose is low in a bacterial cell? |
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Term
| D. Binding of the CAP-cAMP to the lac activator- |
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Definition
| Which of the following is true about the action of CAP at the lac promoter? |
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Term
| E. All of the choices are true. |
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Definition
| Which of the following is true about the regulation of the trp operon? |
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Term
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Definition
| Which of the following is absent in an operon? |
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Term
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Definition
| Which of the following sigma specificity factors is involved in middle gene transcription during SPO1 phage infection of bacteria? |
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Term
| D. Class I genes are the first to be transcribed. |
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Definition
| Which of the following is true about the early phase of T7 infection of bacteria? |
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Term
| Both Correct: T7 phage encodes a new RNA polymerase for phage genes. AND SPO1 phage uses the new sigma factor for phage gene. |
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Definition
| Differences in transcription of T7 and SPO1 genes are |
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Term
| E. none of the choices are correct. |
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Definition
| Which of the following conditions does not lead to a change in gene expression patterns in bacterial cells? |
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Term
| E. antitermination will be affected and transcription from the left promoter will be interrupted |
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Definition
| Predict the outcome of the removal of the N from the DNA sequence of the l phage: |
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Term
| B. It stimulates termination at intrinsic terminator by facilitating hairpin loop formation. |
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Definition
| Which of the following is a possible mechanism for the action of NusA in termination? |
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Term
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Definition
| Which of the following is involved in maintaining lysogeny in l phage? |
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Term
| E. all of the choices are correct. OR1, OR2, OL1, & OL2 |
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Definition
| Which of the following operators is a target of cI gene production? |
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Term
| D. Interaction between repressor and polymerase is essential for activation of transcription and interaction involves region 4 of s-factor. |
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Definition
| Which of the following is true about the interaction with the l repressor and RNA polymerase at PRM? |
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Term
| C. cro repression is important to lysogeny. |
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Definition
| Which of the following statements is true about the cro gene? |
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Term
| D. Coprotease activity in RecA protein is activated and SOS response is induced. |
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Definition
| During an experiment to study the rate of infection of bacteria with T4, the bacterial cultures were accidentally exposed to a UV light source. Which of the following would be expected for this exposure? |
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Term
| E. chromatography and UMP incorporation assay |
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Definition
| You were asked to choose a method to separate a mixture of eukaryotic polymerases. Which of the following methods would you choose? |
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Term
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Definition
| Which of the following are products of RNA polymerase II activity? |
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Term
| D. 7SL RNA and 5S rRNA and U6 snRNA |
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Definition
| Which of the following products are made by RNA polymerase III? |
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Term
| C. There are no repetitive sequences. |
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Definition
| Which of the statements about ribosomal genes is incorrect? |
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Term
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Definition
| An antibody was produced and used in an immunohistochemical assay to detect the location of RNA polymerase I in a thin section of a liver biopsy. The section was antibody-labeled with a fluorescent tag, then viewed under a fluorescent microscope. State where you would expect to find positive staining if the enzyme is present. |
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Term
| B. Splicing function is impaired. |
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Definition
| A new mutant cell line was developed and was found to be defective in polymerase III activity. Which of the following is likely to be observed in this cell line? |
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Term
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Definition
| An experiment is planned to study the effect of reducing the transcription of the myosin gene. Which of the following inhibitors would be useful in this study? |
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Term
| C. in vitro transcription |
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Definition
| Which of the following would you choose to study the role of polymerase subunits in elongation? |
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Term
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Definition
| Which of the following would you choose to specifically collect polymerase subunits in cell extracts? |
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Term
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Definition
| Which of the following is not a part of the core class II promoter? |
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Term
| B. linker scanning mutations |
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Definition
| Which of the following techniques would you use to study the role of the BRE? |
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Term
| C. Promoter can be never within a gene. |
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Definition
| Which statement is wrong regarding promoter in humans? |
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Term
| C. reduction in the amount of rRNA made |
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Definition
| Predict a possible effect of deleting the enhancers region of the polymerase I gene. |
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Term
| E. TFIIA+TFIID, TFIIIIB, TFIIF + pol II, TFIIE, TFIIH |
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Definition
| Which of the following is the correct order of addition of general transcription factors to the initiation complex? |
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Term
| A. Use an antibody to TFIIB and check for a supershift by EMSA. |
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Definition
| An electrophoretic mobility shift assay (EMSA) was conducted to check for the binding of TFIIB to a segment of a DNA promoter region. Which of the following approaches could be used to confirm that TFIIB is indeed binding? |
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Term
| A. a DNA sequence made with multiple cytosines |
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Definition
| You are planning an experiment to look at the specific binding of the TBP to the promoter. Which of the following would be a suitable negative control for this experiment? |
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Term
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Definition
| Which of the following would you expect to bind to a chromatography column made of a resin conjugated to UBF? |
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Term
| E. none of the choices are correct. |
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Definition
| Determine which of the following would most likely lead to a dramatic reduction in transcription. |
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Term
| D. transcription-activation domain and DNA-binding domain |
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Definition
| Which of the following are typical features of transcriptional activators? |
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Term
| E. all of the choices are correct. Zinc fingers, glutamine-rich regions, bZIP motifs, & bHLH motif |
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Definition
| Which of the following can be found in DNA-binding domains? |
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Term
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Definition
| Select the feature that is not true of the GAL4 protein. |
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Term
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Definition
| Which of the following is a target site for GAL4? |
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Term
| C. binding of the activator to DNA, without activating transcription |
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Definition
| One effect of mutation or deleting the activation domain of a transcriptional activator would be |
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Term
| E. All of the choices explanations are possible. |
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Definition
| Which of the following is a reasonable way to explain the ability of enhancers to act at a distance? |
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Term
| A. bending the DNA, causing more activators to bind and interact with each other to enhance transcription. |
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Definition
| HMG I(Y) plays a role in IFN-b transcription by |
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Term
| A. shield genes from activation by blocking enhancer activity |
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Definition
| Insulators are DNA elements that |
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Term
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Definition
| Which of the following histone proteins is the most highly conserved from one organism to another? |
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Term
| A. nucleosome formation, 30 nM fiber formation, radial loop structure |
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Definition
| Which of the following is the correct order of chromatin folding? |
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Term
| A. histones and transcription factors. |
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Definition
| Studying Xenopus transcription of somatic and oocyte 5S rRNA genes, Donald Brown and colleagues demonstrated that the difference in the expression patterns in the two types of cells could be seen as a race between |
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Term
| E. DNase hypersensitivity experiments. |
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Definition
| One method to demonstrate existence of nucleosome free DNA regions is |
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Term
| B. combination of histone modification on a given nucleosome near a gene's control region affects the efficiency of transcription of that gene. |
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Definition
| The Histone Code states that the |
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Term
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Definition
| Which of the following experiments was useful in determining the existence of introns in the adenovirus genome? |
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Term
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Definition
| The first two bases and the last two bases in the splicing signal consensus sequence are |
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Term
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Definition
| Which of the following is the name for the Yeast 40S particle where mRNA splicing takes place? |
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Term
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Definition
| "Snurps“ or snRNPs are composed of |
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Term
| E. Mg2+, U2, and U6 snRNP, and the branch point region of the intron. |
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Definition
| The catalytic center of the spliceosome appears to include |
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Term
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Definition
| Which of the following is the first snRNP to bind during the assembly stage of the spliceosome cycle? |
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Term
| C. U4: base pairs with 3¢ splice site of mRNA. |
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Definition
| Which of the following snRNP is mismatched with its function? |
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Term
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Definition
| The binding of which of the following snRNPs to spliceosome requires ATP? |
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Term
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Definition
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Term
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Definition
| Please put the following steps of Cap synthesis in the correct order. (1) N7 of the capping guanine is methylated. (2) The terminal phosphate is removed from the pre-mRNA. (3) A capping GMP is added to the pre-mRNA. (4) The 2¢-O-methyl group of the penultimate nucleotide is methylated. |
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Term
| E. helps regulate expression of the mRNA from the promoter |
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Definition
| Which of the following is NOT a function of the mRNA Cap? |
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Term
| B. export to the cytoplasm. |
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Definition
| Although snRNA U1 functions in the nucleus, it receives a 5' Cap to enhance its |
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Term
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Definition
| Which of the following is the mRNA polyadenylation signal motif found in mammalian cells? |
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Term
| B. It occurs before the mRNA reaches a chain length of 30 nt. |
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Definition
| When does capping of the pre-mRNA occur? |
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Term
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Definition
| Which of the following is not required for mammalian cell polyadenylation of pre-mRNA? |
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