Term
How do enzymes affect the rate of reaction? 

Definition
They lower the activation energy, so the rate of reaction increases. 


Term
What is the major difference between enzyme catalyzed and uncatalyzed reactions? 

Definition
Catalyzed reactions tend to become saturated 


Term
1. What does reaction order mean?
2. Define:
1st order reactions
2nd order reactons 

Definition
1. Reaction order referes to the number of molecules needed to interact
1st order= only 1 molecule
2nd order = 2 molecules or 2 of the same molecules 


Term
What does a substrate do to an enzyme? 

Definition
Binds to it through conformational changes in the enzyme (referred to as induced fit model)



Term
1. What is Vmax?
2. Why do you hit Vmax? 

Definition
1. Vmax = the maximum theoretical velocity of a reaction
2. You hit Vmax because there is a finite number of enzyme particles 


Term

Definition
Km = Michaelis constant
Km is the [S] where the velocity is half the Vmax



Term
How does Km relate to affinity? 

Definition
1. Low Km = high affinity
2. High Km= low affinity 


Term
1. When is V directly proportional to [S]?
2. Where would you see 1st and zero order reaction? 

Definition
1. V is directly proportional to [S] at low [S]
2. 1st order occurs initially, and zero order is found at Vmax 


Term
Main difference between equilibrium and steady state 

Definition
Equilibrium = [S] and [P] are independent of enzyme activity
Steady State = [S] and [P] depend on enzyme activity/degradation 


Term
What is the steady state assumption? 

Definition
Enzymesubstrate complex does not change over time. (K2 is equal to K1) 


Term
Define:
MicahelisMenten Equation 

Definition


Term

Definition
Kcat is the catalytic constant (turnover rate). This is how much product is made per enzyme per second. 


Term

Definition
The measure of catalytic efficiency. Catalytic perfection is achieved at 10^810^9 

