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Entrobacteriaceae
Micro
18
Health Care
2nd Grade
03/30/2014

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Term
basic characteristics of all enterobacteriaceae
Definition
gram - bacilli facultative anaerobes ferment glucose
all but plesiomonas are oxidase - catalase + mostly opportunistic pathogens normal enteric flora grow on EMB and MacConkey as well as SBA
Term
Escherichia coli
Definition
indole positive; MR positive; Motile
VP negative; Urease negative; Citrate negative
Most common cause of UTI’s
Term
0157: H7 Enterohemorrhagic E. colli
Definition
HUS- hemolytic uremic syndrome causes renal failure most comen in kids
MacConkey with sorbitol can not ferment sorbitol
Term
Klebsiella pneumonia
Definition
indole negative; MR negative; non-motile
VP positive; urease positive; citrate positive Ferments glucose and lactose
Mucoid colonies EncapsulatedCommon cause of pneumonia
Term
serratia marcescens
Definition
motile DNase positive
Chromogenic producing a non-water soluble pigment Nosocomial opportunistic pathogen
Term
proteus mirabilis
Definition
known for swarming
lactose negative
p. mirabilis is indole negative, P. vulgaris is indole positive
Term
salmonella species
Definition
inhabit GI tract of animals motile
negative for lactose fermentation
H2S positive; indole negative; VP negative; urease negative
Always a pathogen Classification of serogroups based on antigens
Term
salmonella species Disease states
Definition
acute gastroenteritis
typhoid fever sever febrile illness fever headache blood cultures only + for few days then moves to inside leukocytes death from dehydration in short time
Term
shigells species
Definition
non-motile H2S negative
Delayed lactose fermenter
least biochemically reactive enterobacteriaceae
Always a pathogen Classification of serogroups based on antigens
shiegellosis dysentery characterized by diarrhea with blood and mucus
Term
Yersinia pestis
Definition
gram negative plump bacillus w bipolar staining safety pin appearance
motile at 25c
grows best at 25-30c causative agent of bubonic plague
Term
campylobacter
Definition
grows best in microaerophilic environment at 42c
asacchrolytic does not utilize carbohydrates causes bacterial diarrhea
Term
helicobacter pylori
Definition
urease positive
colonized the gastric lining causing gastritis and ulcers
associated with gastric cancer
Term
pseudomonas aeruginosa
Definition
non fermenter
oxidase positive
opportunistic/nosocomial
high rate of antimicrobial resistance
isolated from respiratoyr cultures from cystic fibrosis patients
produces the blue pigment pyocyanin
Term
MacConkey's agar
Definition
selective for gram - inhibits gram +
differentiates lactose fermenters
Term
Simmons citrate
Definition
uses citrate as a sole carbon source
ammonium ions as the sole nitrogen source
Term
MTM
Definition
motility test
Term
indole test
Definition
inoculated tube containing tryptophan incubated overnight has kovacs reagent added coller change with in 30 secondes is +
Term
TSI
Definition
contains 1% glucose, sucrose and lactose
if ferments glucose phenol red will turen yellow in slant and but in 8-10 Hr if all 3 ferment full yellow in 16-14hr if h2s is produced butt will turen black
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