Term
| What types of substrates to NAT's work with? |
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Definition
xenobiotic amines, hydrazines
hydroxy gps of N-hydroxylated compounds
endogenous amines and hydroxyls
(Special case- amino gp of cysteine conjugates in cysteine conjugate NAT in Mercapturic Acid pathway) |
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Term
| What is the cosubstrate of NAT? which group is acetylated? |
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Definition
Acetyl CoEnzyme A
thiol group of coenzyme A is acetylated |
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Term
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Definition
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Term
| What are the two most important NATs in the acetylation of xenobiotics? |
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Definition
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Term
| Where is NAT2 mostly found? |
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Definition
Liver
some expression in extrahepatic tissues |
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Term
| Where is NAT1 mostly found? |
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Definition
Blood, intestine, and bladdar
(Low expression in liver) |
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Term
| Expression levels of ______ do not seem to be changed by inducing agents. |
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Definition
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Term
| What do all NATs have in common that allows them to attack Acetyl Coenzmyme A? |
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Definition
| Conserved cysteine residue at the 68 position... this is called the ping-pong bi-bi mechanism where enzyme acetylates itself and then the drug enters and gets acetylated |
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Term
| What is the name of the mechanism for acetylation? |
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Definition
| Ping-Pong Bi-Bi mechanism |
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Term
| What was the first enzyme shown to exhibit polyorphism? |
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Definition
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